FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_2039_d1_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_2039_d1_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255615
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG9250.3618723470844825No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA8230.32196858556813956No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG7450.29145394440858324No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT5260.20577822115290575No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT4140.1619623261545684No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG3820.1494434990121863No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA3290.12870919155761595No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT3010.1177552178080316No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC2910.11384308432603721No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA2780.10875731079944449No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT2750.10758367075484616No Hit
GAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT2730.10680124405844726No Hit
GAGCAAAAGCAGGTAGACATTTAAAGATGAGTCTTCTAACCGAGGTCGAA2700.10562760401384896No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC2680.10484517731745008No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG2610.10210668388005398No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA2560.10015061713905679No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG2560.10015061713905679No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT2560.10015061713905679No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA850.0127.0610661
CGAAAGC2150.087.0713043
AGCGAAA2250.086.401531
GCGAAAG2250.083.201472
CAGGTAC353.5519166E-582.287173
CACCTAC353.5519166E-582.287173
GCAGGGT3500.082.2710659
GCAGGTA2500.080.625659
TTATAGG1500.076.801361
GCAAAAG8900.072.810273
AGGTACT406.885173E-572.0012744
TCTAGCT903.6379788E-1272.0012742
TCCACCT451.2335883E-464.001131
TGCCATG2050.059.696698
AAGCAGG12750.058.7189647
GAGCAAA11100.058.379411
CCTACTA502.0770688E-457.6010175
CCACCTA502.0770688E-457.6010172
AGCTCTA1154.1836756E-1156.348825
CTCTATG1154.1836756E-1156.3377957
Produced by FastQC (version 0.11.2)