FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_2036_d1_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_2036_d1_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505783
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA18150.3588495461492379No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG16360.3234588746557318No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG11280.2230205443836586No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT7240.143144391962561No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG6620.13088617055140248No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT6570.12989760430856712No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA6530.12910675131429883No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT6430.1271296188286281No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC6370.12594333933722565No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA5690.11249883843466467No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC5170.10221774950917685No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACTC100.0072444095143.957679
GAGCGAA1150.0106.44561
GGTCGTT301.6561848E-595.981287
CACCTAC607.2759576E-1295.981283
CCACCTA651.6370905E-1188.615632
CCTACTA651.6370905E-1188.59815
GCAGGTA4050.085.308269
GCAGGGT6700.083.7962659
TCGTTTT353.5607867E-582.261539
TCGCTCC502.4040964E-671.9859545
AGGTCGT406.898954E-571.9859546
AGCGAAA2100.068.57841
TCCACCT851.8007995E-1067.771591
GACAGTA651.4671605E-766.448587
GTAGAAA2350.064.346971
CGATCCC451.2360523E-463.9875225
TTTAAAC808.541974E-962.9877133
CGAAAGC2300.062.5964853
GCGAAAG2400.060.0001642
GCAAAAG19650.058.6145253
Produced by FastQC (version 0.11.2)