FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_2034_d1_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_2034_d1_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363545
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA15090.4150792886712787No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG12560.3454868035593943No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG9830.2703929362252266No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT6310.17356860911303965No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT5200.14303593777936707No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC4620.12708192933474535No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA4580.1259816528902887No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT4430.12185561622357617No Hit
TCCACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT4100.11277833555680865No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG4080.11222819733458032No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC3950.10865229889009614No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG3890.10700188422341113No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT3840.10562653866784029No Hit
GAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT3680.10122543289001361No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCAGA100.0072327275144.029882
CTAAACT100.007247621143.93088
GAGCGAA1300.0127.428571
GCAGGGT6500.096.322939
GCAGGTA3550.091.2237559
CAATTCG608.767529E-1083.959649
GCAAAAG13900.077.153393
CTACACT1350.069.3095554
AGCAGGG10550.068.89588
AGCGAAA2450.067.605862
AAGCAGG18600.066.935567
GACAGTA451.2373339E-463.9692467
GCGAAAG2650.062.4691353
TCTACAC1500.062.3785973
AGTAGAA1850.062.2917631
TCCACCT1051.6370905E-1161.7355841
CGAAAGC2750.060.197534
GAGCAAA18100.059.6891021
CCTACTA1102.7284841E-1158.8888855
AGCAAAA18900.057.5357442
Produced by FastQC (version 0.11.2)