HVHNVAFXX_n02_2032_d5_nw.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HVHNVAFXX_n02_2032_d5_nw.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	144507
Sequences flagged as poor quality	0
Sequence length	150
%GC	44

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

$Per base sequence content$

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA	758	0.5245420637062564	No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG	746	0.5162379677109068	No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA	711	0.4920176877244701	No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT	667	0.46156933574152115	No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC	472	0.3266277758170884	No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG	422	0.2920273758364647	No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG	394	0.2726511518473153	No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG	314	0.2172905118783173	No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC	282	0.1951462558907181	No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT	268	0.18545814389614343	No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA	257	0.17784605590040622	No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG	246	0.17023396790466896	No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC	240	0.16608191990699414	No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT	237	0.1640058959081567	No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA	234	0.16192987190931926	No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC	218	0.15085774391551968	No Hit
TCCACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT	209	0.14462967191900739	No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT	203	0.14047762392133253	No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA	199	0.13770959192288262	No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT	197	0.13632557592365765	No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT	196	0.1356335679240452	No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA	194	0.13424955192482024	No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT	194	0.13424955192482024	No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT	185	0.12802147992830795	No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC	172	0.11902537593334579	No Hit
GTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATC	168	0.11625734393489588	No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG	167	0.11556533593528341	No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC	165	0.11418131993605847	No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC	161	0.11141328793760855	No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT	159	0.1100292719383836	No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA	156	0.10795324793954617	No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG	155	0.10726123993993371	No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC	155	0.10726123993993371	No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA	153	0.10587722394070877	No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG	152	0.10518521594109628	No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG	151	0.10449320794148381	No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG	148	0.10241718394264639	No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT	148	0.10241718394264639	No Hit
GAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA	146	0.10103316794342143	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGAGCGA	10	0.00723237	144.00243	3
GAGCGAA	90	0.0	128.00215	1
GAGCAAA	615	0.0	100.684616	1
CACCTAC	55	4.3655746E-10	91.6379	3
GCAGGTA	225	0.0	86.40145	9
CCACCTA	60	8.6947693E-10	84.00142	2
AGTAGAA	115	0.0	81.39268	1
AGCAAAA	785	0.0	78.88031	2
TCCACCT	65	1.6389095E-9	77.539764	1
CCTACTA	75	5.820766E-11	76.80129	5
GCAGGGT	300	0.0	76.80129	9
AGCGAAA	190	0.0	75.79075	1
AAGCAGG	970	0.0	74.97034	7
GTAGAAA	205	0.0	73.75734	1
ACTATAT	30	0.0019860305	72.00121	3
CTAGTGC	90	3.6379788E-12	72.00121	9
GCGAAAG	205	0.0	70.24508	2
CGAAAGC	215	0.0	66.97787	3
CAAAAGC	920	0.0	66.522865	4
GCAAAAG	920	0.0	66.522865	3

Summary