FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_2032_d1_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_2032_d1_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384762
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA13320.346188033121774No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG12330.32045784147083134No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG11060.2874504238984099No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT8550.22221529153086841No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT7190.18686876562654317No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG5030.1307301656608501No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT4780.12423264251667264No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA4610.11981432677863199No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC4590.1192945249270978No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC4510.11721531752096101No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT4400.11435640733752295No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA4180.1086385869706468No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA4120.1070791814160442No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT3940.10240096475223645No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1350.0127.983141
CACCTAC700.092.54723
GCAGGTA3300.091.600479
GCAGGGT5600.082.253499
CCACCTA851.8189894E-1276.225252
CCTACTA851.8189894E-1276.205445
ATCACAC406.89914E-571.981153
GCAAAAG13850.070.6818543
TTATAGG1050.068.5623861
AAGCAGG18300.062.5328757
AGCGAAA2800.061.706151
GTAATTA350.00366771661.698133
CAAGGTA702.4516157E-761.6901174
GGTAGTT607.09885E-659.97657
AGCAGGG10450.059.9191068
TCCACCT1102.7284841E-1158.9013251
AGTAGAA1600.058.4922871
AGATCAC502.0799307E-457.592411
TCTAGCT1007.6397555E-1057.592412
CGAAAGC3150.054.842783
Produced by FastQC (version 0.11.2)