FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_2032_d-2_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_2032_d-2_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365711
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA6960.19031420985422914No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG5680.15531389539827897No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT5080.13890749799705232No Hit
GGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCTCGGAGCAGGAA4400.12031358094232879No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC3750.082.528333
AGCGAAA4050.078.20291
GAGCGAA1800.071.982221
GCGAAAG4300.071.9723742
GAGCAAA6200.042.957131
GCAAAAG7550.033.3646773
CAGCTCG3800.032.1937689
TATACAG4550.030.0502935
CAAAAGC8250.029.657294
AGCAAAA8550.029.4623782
GAAAGCA11350.029.1654344
GCTAGTC1050.00798161627.4180492
TATACAT5150.026.5492864
AAAGCAG17150.026.4352195
GTATATA9300.026.3160821
CATACAT5250.026.0435858
GTGTAGG1400.001040874225.7079351
TACATAC4800.025.4867326
AAGCAGG20900.023.7579676
ATATACA9100.022.9362533
Produced by FastQC (version 0.11.2)