FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_2031_d-2_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_2031_d-2_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255149
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG4580.17950295709565783No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA4450.17440789499468937No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2100.085.746161
GCGAAAG2750.068.057992
CGAAAGC3100.060.3740273
GAGCGAA1051.1696102E-954.8775441
GTTCCGA400.00621885853.9883086
GAGCAAA3550.048.694161
AGTTCCG450.00990635847.9896135
GCAAAAG4700.038.289583
AGCAAAA5000.034.5525172
GTACTAT1051.92242E-434.2984661
GTCTAGG850.00282750233.8949551
ATACCAT850.002835672633.8750156
CAGCTCG2653.6379788E-1232.5839359
CAAAAGC5700.031.572114
GAGTAGG1652.5017744E-630.5568141
AGAGTCG1204.22256E-429.9935075
GTATTAT2701.8371793E-1029.3442421
ATGCGCC200.006278574528.80506125-29
ACTAGAG1255.362582E-428.7937643
CATACAT3701.0913936E-1125.2819048
Produced by FastQC (version 0.11.2)