FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_2030_d1_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_2030_d1_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387495
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA17570.45342520548652243No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG14360.3705854269087343No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG11460.29574575155808464No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT6570.16955057484612707No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC4790.12361449825159035No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA4740.12232415902140673No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT4710.12154995548329657No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT4620.11922734486896605No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC4360.11251758087201125No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT4240.10942076671957059No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT4050.10451747764487283No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1250.0138.254141
GCAGGTA3700.089.4649059
CCACCTA608.731149E-1084.008592
GCAGGGT5650.081.513239
AGCGAAA2750.081.1719361
GCGAAAG3000.074.407612
CGAAAGC3000.074.38843
GTATAAA300.001987888772.007361
GATAGCA903.6379788E-1271.988776
CAATTCG502.408009E-671.96099
GCAAAAG14250.070.725813
CCTACTA755.124093E-967.189525
GGATAGC1001.0913936E-1164.7898945
CACCTAC808.532879E-962.990173
TAGCAAA1051.6370905E-1161.680778
GTAGAAA2000.061.206261
AAGCAGG19550.060.366187
AGCAGGG10700.057.8377348
CAGGTAC400.00621979653.9915733
AGCTCTA1600.053.9915735
Produced by FastQC (version 0.11.2)