FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_2030_d-2_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_2030_d-2_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148335
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATAC300.001987596571.987872
TAGGGAT753.9269617E-757.590294
GTGCTAC400.00621256953.99091
CGAAAGC901.3817789E-647.9919052
GAGCGAA450.00989637547.9919051
CCATTCG450.00989637547.9919059
AGCGAAA657.576276E-444.3002241
GAGTATA750.001531750338.3935241
CAATACT750.001531750338.3935244
GTATTAC800.00210294835.993931
GGTTTAC800.00210294835.993931
GTGTACT800.00210294835.993931
TAGAATA850.002831200433.876644
GCGAAAG1301.7271228E-533.2251661
CCTGTAC950.004880788730.3106783
ATATTAG1453.642647E-529.7880821
ACCTATC1050.00795822327.4239468
ACCGTGG1100.00998463926.1774068
ATAGAAG1450.001273054924.82346
GTAATAC1500.001551014423.9959533
Produced by FastQC (version 0.11.2)