FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_2029_d3_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_2029_d3_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239916
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA11700.48767068473965886No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG10580.44098767902099073No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG7750.3230297270711416No Hit
TCCACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT4510.18798246052785142No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT4480.1867320228746728No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG4390.18298070991513696No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT3790.1579719568515647No Hit
GTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATC3720.15505426899414795No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC3550.1479684556261358No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT3170.13212957868587338No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA3050.12712782807315895No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC3030.12629420297103985No Hit
GGGTTTCGACTTTCTCTTACTTGATCCATCATTGCCCTCTGGGCAGCTGT2970.12379332766468264No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG2860.11920838960302774No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT2830.11795795194984912No Hit
GAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT2760.11504026409243236No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC2710.11295620133713466No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG2660.11087213858183696No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT2620.10920488837759884No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT2570.10712082562230114No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC2530.105453575418063No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG2520.10503676286700346No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT2490.10378632521382485No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG2430.10128544990746763No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT2430.10128544990746763No Hit
AGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTAT2420.10086863735640807No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTT151.2637136E-4143.98529
AGGTCGT151.2637136E-4143.98526
GAGCGAA950.0136.407031
GGTCGTT203.9718652E-4107.988917
GCAGGTA2500.095.030249
GCAGGGT4550.094.935299
CCTACTA1350.085.324565
AGCGAAA1550.083.604312
TCCACCT1350.079.991781
CCACCTA1350.079.991782
CACCTAC1400.077.1349263
CGAAAGC1700.076.227464
GCGAAAG1700.076.227463
TTATAGG1050.075.420821
GAGCAAA11050.072.318351
CGATCCC300.001988580771.99265
AGCAGGG7600.071.99268
AAGCAGG13050.070.061767
GCAAAAG11750.066.784633
CAAGGTA554.224683E-665.4478154
Produced by FastQC (version 0.11.2)