FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_2029_d-2_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_2029_d-2_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479402
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA9630.20087525709112605No Hit
GGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCTCGGAGCAGGAA9330.1946174609200629No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG8220.17146361508712937No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT6150.12828482150679388No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA5400.1126403310791361No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5650.072.62781
GCGAAAG6500.064.231162
GAGCGAA3200.062.991881
CGAAAGC6700.061.239443
GAGCAAA8800.059.7195661
GCAAAAG10900.051.5109143
TCGTGCA450.00992455447.973799
GTCGTGC657.60743E-444.2834978
AGCAAAA12650.043.2468262
CAAAAGC13100.042.3107384
CAGCTCG4450.035.576079
AAAAGCA17300.031.6226795
AAAGCAG25400.029.7568366
TATACAG6050.028.5553195
ACAGCTC7400.027.2283658
GTATAGG1607.156117E-526.996521
TATAGAC7200.026.9937022
TACAGCT6700.026.8509987
TAGATAG1358.421135E-426.6604465
CTAGGGT1701.0815092E-425.4058384
Produced by FastQC (version 0.11.2)