FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_reproductive_ifn--_red_needle_f10_pcr1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_reproductive_ifn--_red_needle_f10_pcr1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887648
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTGAAGCGAGAGCTAACAACAGTATCAACAGGTTTAATTTGGATTTG53480.6024910775442518No Hit
GTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAACAGGTTTAATTTGG37650.4241546198493097No Hit
GTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCC35130.3957649879231407No Hit
GTATCAACAGGTTTAATTTGGATTTGGAAACGAGAGTTTCTGGTCATGAA34030.3833726882728289No Hit
GCTAACAACAGTATCAACAGGTTTAATTTGGATTTGGAAACGAGAGTTTC23780.2678989869858322No Hit
GCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTG20730.23353851977360396No Hit
GTATTACCTGACCATGAACAATAAGCATTGGTTGGTGCACAAAGAGTGGT19180.2160766429936191No Hit
GTCAATATGCTAAAACGCGGAGTAGCCCGTGTAAACCCCTTGGGAGGTTT19020.21427412668084647No Hit
CCTTAAACCTGTCTTGTAACCTTGATACTTACTTTCAGCCTCCATGTGTC18630.2098804931684632No Hit
GGTAATACAGATCTGAAAAGTCAAGGCCTGTCCTTGGTTCACAGTCAAGT18240.2054868596560799No Hit
CAATGAGTCGGTCGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGC18210.20514888784743504No Hit
ATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCG18150.20447294423014528No Hit
CAGTATCAACAGGTTTAATTTGGATTTGGAAACGAGAGTTTCTGGTCATG18070.20357168607375897No Hit
ATCTAAGCTTGTCCATTTTTAGGCGGCATTTCAAGTGGCCAGAGAACAGC17800.20052993979595515No Hit
CAACAGTATCAACAGGTTTAATTTGGATTTGGAAACGAGAGTTTCTGGTC17550.19771350805724794No Hit
ATGCTGGATGAGGGAGTGGAACCAGATGATGTCGATTGCTGGTGCAACAC17370.19568567720537872No Hit
GGTTTAAGGAGACCAATAGAAACTGGGCTTGTCGAGACAGAGAAGACTCT17340.19534770539673382No Hit
GTCATAGGAGTTGGGGACAAGAAAATCACCCACCACTGGCATAGGAGTGG17100.1926439309275749No Hit
GGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAA16630.1873490392588053No Hit
CTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTGA16420.18498323659829122No Hit
GGTTTAATTTGGATTTGGAAACGAGAGTTTCTGGTCATGAAAAACCCAAA16330.18396932117235662No Hit
CTACATGTACTTGGATAGGAGCGATGCCGGGAAGGCCATTTCGTTTGCTA16190.18239211939868055No Hit
ATGCTAAAACGCGGAGTAGCCCGTGTAAACCCCTTGGGAGGTTTGAAGAG15110.1702251342874653No Hit
GTATATGACTTTTTGGCTCGTCGAGCTTCCCAAAAGCCAGGCAATGGCAA14910.16797198889649953No Hit
TTCATATCCTGTATCATTGACAATCATCCCGCTATGCTGGGAGCCATGCA14400.16222646814953676No Hit
GAGTAGCCCGTGTAAACCCCTTGGGAGGTTTGAAGAGGTTGCCAGCCGGA14260.16064926637586072No Hit
GAATATGACACGCCCTTCAATCTAAGCTTGTCCATTTTTAGGCGGCATTT13870.15625563286347743No Hit
TCCTTGTAGAGTGAGAAGGGAGCGTCACGGCTCTTCTAGATCGCCGTGCC13580.15298857204657704No Hit
TCTTAGAACACGTAAACTTGGCACATGTCACCAAGCTCCCTTTGCCAAAA13530.15242528569883557No Hit
GTCTTTAGCTGGTCCACTCAGCCACCACAACACCAGAGAGGGTTACAGAA13270.14949619669058006No Hit
GTGTAAACCCCTTGGGAGGTTTGAAGAGGTTGCCAGCCGGACTTCTGCTG13090.14746836583871084No Hit
GTCCATGACCCAGCAGAAGTCCGGCTGGCAACCTCTTCAAACCTCCCAAG12960.14600382133458306No Hit
TAATAAAGAAGTTCAAGAAAGATCTTGCTGCCATGTTGAGAATAATCAAT12750.143638018674069No Hit
CCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATT12670.14273676051768266No Hit
TCCGTACACAACCCAAGTTGATGTCGTGTTGCACCAGCAATCGACATCAT12430.14003298604852374No Hit
TCCTAGAGGAGAATGGAGTTCAACTGACAGTTGTTGTGGGATCTGTAAAA12410.13980767150942716No Hit
CTCCAAGAGAGAAGTGGTCCATATGATCGGTTGACCCCGCTGTCACCATT12370.139357042431234No Hit
ATCAACAGGTTTAATTTGGATTTGGAAACGAGAGTTTCTGGTCATGAAAA12360.1392443851616857No Hit
GTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACAT12340.1390190706225891No Hit
AGTATCAACAGGTTTAATTTGGATTTGGAAACGAGAGTTTCTGGTCATGA12320.13879375608349254No Hit
GAGTTATGAGTGCCCTATGCTGGATGAGGGAGTGGAACCAGATGATGTCG11940.13451277984065757No Hit
CCCCTTGGGAGGTTTGAAGAGGTTGCCAGCCGGACTTCTGCTGGGTCATG11720.1320343199105952No Hit
GTAGTATGCACTCCCGCGTCTAGTGATCTCTGCTGCCATGGCTGTAGTCA11640.13113306175420888No Hit
CGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAACAGGTTTAA11620.1309077472151123No Hit
CATATTGAGTGTCTGATTGCTTGTCAAGGTAGGCCTCACCTTGTGTTGGG11530.12989383178917768No Hit
GGATAGGAGCGATGCCGGGAAGGCCATTTCGTTTGCTACCACATTGGGAG11510.1296685172500811No Hit
CTCAATATGTCTGCAAAAGAACATTAGTGGACAGAGGTTGGGGAAACGGT11380.12820397274595335No Hit
CTACAAGGAAGTTGCAAACGCGGTCGCAGACCTGGTTAGAATCAAGAGAA11370.12809131547640507No Hit
ATCTAGAAGAGCCGTGACGCTCCCTTCTCACTCTACAAGGAAGTTGCAAA11320.12752802912866362No Hit
GTTAGTAACATGGCCGAGGTAAGATCCTATTGCTACGAGGCATCGATATC11210.12628879916363245No Hit
GTACAAGTACCATCCTGACTCCCCCCGCAGATTGGCAGCAGCAGTCAAGC11000.12392299650311836No Hit
AGCTTAGATTGAAGGGCGTGTCATATTCCTTGTGCACTGCGGCATTCACA10950.1233597101553769No Hit
GTCATGGACCGTCAATCACGCTTTCCGCCGCCTGACGCGTCTCCGCCAGC10880.12257110926853888No Hit
TTAGTGGACAGAGGTTGGGGAAACGGTTGTGGACTTTTTGGCAAAGGGAG10860.1223457947294423No Hit
TCCCTTAACAGCTGTTCCTATGACGGCTGGGTCACATTCTAATGAGTAAT10830.12200782292079743No Hit
TTATAGATGAATACCCCCGTGCCACATCTCGTTTCCTTTTTTGAGAAGTC10810.12178250838170085No Hit
TCCATGCTCTGTGCTCAAGCGGACATTCCTTCAGTGTGTCACCGTCGACA10800.12166985111215257No Hit
GAAGAAATCCGGAGGATTCCGGATTGTCAATATGCTAAAACGCGGAGTAG10720.12076859295576624No Hit
TCTCACAGTGGCCTCAAATGCCTTTCCGATGGTGCTACCACTCCTATGCC10700.12054327841666966No Hit
CTTCTAGATCGCCGTGCCTCACCTTTTTTGTGATGACAGGTTCCGTACAC10640.11986733479937993No Hit
TTCCTTCAGTGTGTCACCGTCGACAACAAAACTGTTGTTGGTCTTTGCCG10530.11862810483434875No Hit
GTGCGTGGCACGGCCATTGCTCGAATTGCCAACCAGGCCAAAGCAAATCC10460.11783950394751072No Hit
GTACAGATCATGGACCTCGGGCACATGTGTGACGCCACCATGAGTTATGA10430.11750153213886586No Hit
ATATCGGACATGGCTTCGGACAGTCGTTGCCCAACACAAGGTGAGGCCTA10330.11637495944338296No Hit
GTCATATTCCTTGTGCACTGCGGCATTCACATTCACCAAGGTCCCAGCTG10170.11457244313061034No Hit
TTCTTACATTGTCATAGGAGTTGGGGACAAGAAAATCACCCACCACTGGC10140.11423447132196546No Hit
TCTCTCCACAGAGGGCCCACCTGATTGAGATGAAAACATGTGAATGGCCA10090.11367118497422402No Hit
TCTCCATTCCATACCAGCAGCCGTCTTTTGCTCGAAACGATAGTGGGGGC10050.11322055589603086No Hit
GCTAAAGACTTGGGTATGATAAGATCACTTTCTTCTACTCCATCTGTCCA9930.11186866866145137No Hit
ACTCTATGTCAACTGTTGGCTTGTCCTGTGCCATCACGGTAACGCAGCCT9880.11130538231370994No Hit
GTTCTAAGAAGATGACCGGGAAGAGCATTCAACCGGAAAATCTGGAGTAT9840.11085475323551679No Hit
ATCCAGCATAGGGCACTCATAACTCATGGTGGCGTCACACATGTGCCCGA9740.1097281805400339No Hit
CCCTACAGCACCATTCCTCAATGACCCTTCCACTTGCAGTAGTTGATCTC9690.10916489419229244No Hit
GTATCAGGTGCATTGGAGTCAGCAATAGAGACTTCGTGGAGGGCATGTCA9630.1084889505750027No Hit
TCCCAGGCCTGCTTGACTGCTGCTGCCAATCTGCGGGGGGAGTCAGGATG9550.10758769241861639No Hit
ATCATGACCAAGTATATGACTTTTTGGCTCGTCGAGCTTCCCAAAAGCCA9500.10702440607087493No Hit
CTCTTGGAGTGCTTGTGATTCTACTCATGGTGCAGGAGGGGTTGAAGAAG9320.10499657521900574No Hit
TCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGG9280.10454594614081258No Hit
GTTGAGAATAATCAATGCTAGGAAAGAGAGGAAGAGACGTGGCGCAGACA9260.10432063160171601No Hit
CACTAGCAGCGTGCCTATGAGGATCTGTGAGAACCAGGACATTCCTCCAA9230.10398265979307113No Hit
GTCCACAATGTGTGAGACTTTGGCCATTCACATGTTTTCATCTCAATCAG9220.10387000252352284No Hit
CCATTAATGAGGACCATCAAGTCACCTTCAAGAGCAGAGATCGCAGTTTG9190.10353203071487796No Hit
GATCTGTACGTGGCACTTGTTCACTCCCAATGTGGTAGCAAACGAAATGG9170.10330671617578138No Hit
CTCCTGCACCATGAGTAGAATCACAAGCACTCCAAGAGAGAAGTGGTCCA9150.1030814016366848No Hit
AATGAGGACCATCAAGTCACCTTCAAGAGCAGAGATCGCAGTTTGCAGAA9130.10285608709758823No Hit
ATCCTAGAGGAGAATGGAGTTCAACTGACAGTTGTTGTGGGATCTGTAAA9010.10150419986300876No Hit
CTGTATGCCGGGGCAATCAGCAGTATCATGACCAAGTATATGACTTTTTG9000.10139154259346046No Hit
ATTCCGGATTGTCAATATGCTAAAACGCGGAGTAGCCCGTGTAAACCCCT8970.10105357078481561No Hit
GTGCAGGAGGGGTTGAAGAAGAGAATGACCACAAAGATCATCATGAGCAC8950.10082825624571903No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAG2250.0102.425281
TTCGAGA5600.079.71152
GTTCGAG12700.072.584851
TGACGAC608.427014E-871.99756
GAGATGC6450.071.439375
TCGAGAT6350.071.430593
CGACAGT4250.067.781441
CGAGTCT7300.067.066164
TTCGAGT7200.066.9976652
CAGTGCG2650.065.205273
ACAGTTC4450.064.716853
AGATGCT7150.064.4453056
TCGAGTC7600.064.418813
AGTGCGT2750.062.8341754
AGTCTGA12850.060.528561
GTGACGA607.091452E-659.9979135
GAGTCTG8250.059.343395
GTCAGTG2950.058.5907331
GTCTGAA13700.057.2826772
CTGAAGC14050.055.343274
Produced by FastQC (version 0.11.2)