FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_ny238_plasmid_mix_cb10-14_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_ny238_plasmid_mix_cb10-14_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613395
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT10880.17737347060214056No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT10850.17688438934128906No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG9860.16074470773318986No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG8520.13889907808182328No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG8270.13482340090806086No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA7970.129932588299546No Hit
TCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTG7790.126998100734437No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG7480.12194426103897163No Hit
GTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACT7380.12031399016946666No Hit
CTTTATAGCCAACCTTTGTTCATGGCAGCCAGCATATGGGCATATGTTGC7310.11917280056081318No Hit
CCCATATGTCCTTCCGAGTGAGAGACACAAAAAATTCCAACACACTATTG7030.11460804212619925No Hit
GCATTAATGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGC6980.11379290669144679No Hit
TTGCAATAGTGTGTTGGAATTTTTTGTGTCTCTCACTCGGAAGGACATAT6870.11199960873499132No Hit
TTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCC6540.10661971486562492No Hit
GTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC6340.10335917312661498No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC6290.1025440376918625No Hit
GCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAG6270.10221798351796152No Hit
ACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATA6210.10123982099625854No Hit
GGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG6150.10026165847455554No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGACA350.003665511761.711914
AGGCCGT657.5900933E-444.3059847
CGAGGCT1105.5184883E-639.2712145
TAGCACA1157.487284E-637.563774
TACAAAA1406.9738053E-735.9986152
GAAGGGT800.00210674235.9986156
TATAAGG1854.471076E-935.025682
TTAACCA850.002836289633.881054
GGAATGT1957.4742275E-933.2294928
CTCGATG1102.5365848E-432.7260139
GTATTGG1352.2439293E-532.0013771
TCTATGT1352.2451806E-531.998775
ACTACAA900.003752869331.9987688
GGGCCAC900.003752869331.9987689
AGCGGCG1402.8785955E-530.8559557
CCTTCGC1902.2048152E-730.3146252
TATGTAC950.004889498530.3146253
GAATGTC1204.22501E-429.9988467
AAACCGG1204.22501E-429.9988466
GACACTC1703.1768795E-629.6459187
Produced by FastQC (version 0.11.2)