FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_negative_control_te_pcr2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_negative_control_te_pcr2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length150
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTTGCTGGTGCGACCCCGTCCAGCCGGCGACGGGCCCGGTCCCGCCG11.7241379310344827No Hit
GAGTTATGAGTGCCCTATGCTGGATGAGGGAGTGGAACCAGATGATGTCG11.7241379310344827No Hit
GACGCTCCCTTCTCACTCTACAAGGAAGTTGCAAACGCGGTCGCAGACCT11.7241379310344827No Hit
CTGCTAGTGTGGTTAGGTTTGAACACAAAGAATGGATCTATCTCCCTCAC11.7241379310344827No Hit
ATGAAGGAGACCAGCAAGGCTGGTCTGACTTTAAAGCCCGCTACCAATGC11.7241379310344827No Hit
GCTCTAACACCACTAGCTCGAGGCACACTGCTCGTGGCATGGAGAGCGGG11.7241379310344827No Hit
GCCAAAAAGTCCACAACCGTTTCCCCAACCTCTGTCCACTAATGTTCTTT11.7241379310344827No Hit
GCTGTTCTCTGGCCACTTGAAATGCCGCCTAAAAATGGACAAGCTTAGAT11.7241379310344827No Hit
GCACAGAGCATGGAATAGTTTTCTTGTGGAGGATCACGGGTTTGGAGTCT11.7241379310344827No Hit
TATTTTGTTAGGGCGGCAAAGACCAACAACAGTTTTGTTGTCGACGGTGA11.7241379310344827No Hit
GTAGAAGAAAGTGATCTTATCATACCCAAGTCTTTAGCTGGTCCACTCAG11.7241379310344827No Hit
CCATATGATCGGTTGACCCCGCTGTCACCATTGACCTCACTAAGTTGCTC11.7241379310344827No Hit
CCTAATTACAATCTCTACATCATGGATGAAGCCCACTTCACAGACCCCTC11.7241379310344827No Hit
GCTCCTCAGTGCGGCTCCTTTTGTAACGTGCCACATGGTGTGGAAGCCTC11.7241379310344827No Hit
GCCATTGCTCGAATTGCCAACCAGGCCAAAGCAAATCCATTAATGAGGAC11.7241379310344827No Hit
TCTCTGAGATGTCAGTTGTTATGACAAAGTCCCACTCTTGATTTTTTGTT11.7241379310344827No Hit
GAAGGGGAGCTCAATGCTATCCTAGAGGAGAATGGAGTTCAACTGACACT11.7241379310344827No Hit
ATCATGACTACCAGCACTGCCATTGATGTGCTCATGATGATCTTTGTGGT11.7241379310344827No Hit
GTGAATGAGCTGCCCCATGGCTGGAAAGCCTGGGGGAAATCGTATTTTGT11.7241379310344827No Hit
GACCGGAGATATATGTGCTCAATATGATCTATGTTTTAATTCAATTCATC11.7241379310344827No Hit
CATTAATGGATTTGCTTTGGCCTGGTTGGCAATTCGAGCAATGGCCGTGC11.7241379310344827No Hit
GGTAATACAGATCTGAAAAGTCAAGGCCTGTCCTTGGTTCACAGTCAAGT11.7241379310344827No Hit
TAACATGGCCGAGGTAAGATCCTATTGCTACGAGGCATCGATATCGGACA11.7241379310344827No Hit
ATGTTGTAGTATGAGTAAACTGATATTAGCAAGCCAATACAAAAAACCGA11.7241379310344827No Hit
CTCCTGCACCATGAGTAGAATCACAAGCACTCCAAGAGAGCTCTCTTATA11.7241379310344827No Hit
CTGTTACTCACAAGGAGTGGGAAGCGGAGCTGGCCCCCTAGTGAAGTTCT11.7241379310344827No Hit
CATTGATGTGCTCATGATGATCTTTGTGGTCATTCTCTTCTTCAACCGGT11.7241379310344827No Hit
TTCCCCTTCTGTGTCCATGATTGGTGAGTTAGAGTCAGGAAACGCATCAC11.7241379310344827No Hit
GGTTAAAAACACAAAGTCAGCAACAGCGGGCAGGAGCTACAGCACGGAGC11.7241379310344827No Hit
TTTCAACCCTTGTTGATATGTATCCTCTTGCAGCTATACTTGAGGGGTCT11.7241379310344827No Hit
GAAAATGAAGGAGACCAGCAAGGCTGGTCTGACTTTAAATGCCGCTACAA11.7241379310344827No Hit
GTGGAGGGATCATGCTCCTCTCCCTGAAAGGGAAAGGTAGTGTGAAGAAG11.7241379310344827No Hit
ATTCCATCCACAACGGGATTCTTCATGATGCCAGCTCCAGAACAGAAAGT11.7241379310344827No Hit
GTAATGGAGGCCCTGGGAACACTGCCAGGACACATGACAGAGAGGTTTCA11.7241379310344827No Hit
TTCCTGGGCCTTATCTCCATTCCATACCAGCAGCCGTCTTTTGCTCGAAA11.7241379310344827No Hit
GATACGTGCACTGGCCGGAGGGTTTGCCAAGGCAGACATTGAGATGGCTG11.7241379310344827No Hit
ATGGTCTGTCACCCAATCAAAGCCTGAGCTCCAGGCTCTCTCTGGGACTT11.7241379310344827No Hit
GAGTAGAATCACAAGCACTCCAAGAGAGAAGTGGTCCATATGATCGGTTG11.7241379310344827No Hit
CTATTGGTCTCCTTAAACCTGTATTTTAAACTTGATACTTACTTTCAGCC11.7241379310344827No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT11.7241379310344827No Hit
TAATCAATGCTAGGAAAGAGAGGAAGAGACGTGGCGCAGACACCAGCATC11.7241379310344827No Hit
GTCCACAATGTGTGAGACTTTGGCCATTCACATGTTTTCATCTCAATCAG11.7241379310344827No Hit
CCAACTTGTGTGGCCATCGCCATTAAGGAGTAGTTGTTGTATGAAGTGGT11.7241379310344827No Hit
TCTCCTACAGTGTTCATTTCTGCGAAAGTAGCACCCATCAGGATCACAAG11.7241379310344827No Hit
GTACATTGAAAGAGCAGGTGACATCACATGGGAAAAGGACGCGGAAGTCA11.7241379310344827No Hit
GGTTTGAAGAGGTTGCCAGCCGGACTTCTGCTGGGTCATGGACGCATCAG11.7241379310344827No Hit
GCATTGGTAGAATTCAAGGATGCCCCCGCCACGAGGCAAACCGTCGTCGT11.7241379310344827No Hit
CTGAAGAGCGGTGAGGGAAGACTTGATCCATACTGGGGGGATGTCAAGCA11.7241379310344827No Hit
ATCGTTTCGAGCAAAAGACGGCTGCTGGTATGGAATGGAGATAAGGCCCA11.7241379310344827No Hit
GAGATGCCTAAAACCAGTCATACTTGATGGTGAGAGAGTCATCTTGGCTG11.7241379310344827No Hit
TTGCTGCAATGACGAGAGGATCACTTGAATCGCTGCATCTGCACTCCCAT11.7241379310344827No Hit
AACTTCCTTGTAGAGTGAGAAGGGAGCGTCACGGCTCTTCTAGATCGCCG11.7241379310344827No Hit
AGACATGCGGAACTAGAGGACCATCTCTGAGATCAACTACTGCAAGTGGA11.7241379310344827No Hit
GTCCTCAGCTTAAACTCTCCCTCAATGGCGGCTACCTTATCGGCCTCAGG11.7241379310344827No Hit
AATTACAATCTCTACATCATGGATGAAGCCCACTTCACAGACCCCTCAAG11.7241379310344827No Hit
GTCATGGACCCATCAGAAAGGTTTTGGCGATACTAGCCATTTTGAGATTT11.7241379310344827No Hit
ATACTGCTGATTGCCCCGGCATACAGTATCAGGTGCATTGGAGTCAGCAA11.7241379310344827No Hit
GAGTAGAAGAAAGTGATCTTATCATACCAAAGACTATAGCAGGTCCACTC11.7241379310344827No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGAA50.0144.04
GTACATT50.0144.01
TACATTG50.0144.02
ACATTGA50.0144.03
ATTGAAA50.0144.05
TTGAAAG50.0144.06
AAAGAGC50.0144.09
TGAAAGA50.0144.07
GAAAGAG50.0144.08
Produced by FastQC (version 0.11.2)