FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_negative_control_te_pcr1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_negative_control_te_pcr1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT32.9702970297029703No Hit
CCCCAGGGCCAAGCATGTGAGGGAGATAGATCCATTCTTTGTGTTCAAAC21.9801980198019802No Hit
TCCCTTAACAGCTGTTCCTATGACGGCTGGGTCACATTCTAATGAGTAAT21.9801980198019802No Hit
ACCTTGACAAGCAATCAGACACTCAATATGTCTGCAAAAGAACATTAGTG21.9801980198019802No Hit
TCGGGGGTGTGTTCAACTCACTGGGTAAGGGCATTCACCAGATTTTTGGA10.9900990099009901No Hit
ACTGTGAGAGGCGCCAAGAGAATGGCAGTCCTGGGGGATACAGCCTGGGA10.9900990099009901No Hit
AAGCACTTGATCAAGGTTGAAAACTGGATATTCAGCAACCCCGGGTTTGC10.9900990099009901No Hit
CTCTTGGAGTGCTTGTGATTCTACTCATGGTGCAGGAGGGGTTGAAGAAG10.9900990099009901No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC10.9900990099009901No Hit
AAAGTGATCTTATCATACCCAAGTCTTTAGCTGGTCCACTCAGCCACCAC10.9900990099009901No Hit
CCTAGAGGAGAATGGAGTTCAACTGACAGTTGTTGTGGGACTGTCTCTTA10.9900990099009901No Hit
GTATTCATCTATAATGATGTTGAAGCCTGGAGGGACCGGTACAAGTACCA10.9900990099009901No Hit
GATAGAATTATCCGCTACTCCAGATTTTCCGGTGGACTGCTATTCCCGGT10.9900990099009901No Hit
ACACGAAGCACTTGATCAAGGTTGAAAACTGGATATTCAGGAACCCCGGG10.9900990099009901No Hit
GAATATGACACGCCCTTCAATCTAAGCTTGTCCATTTTTAGGCGGCTTTT10.9900990099009901No Hit
CTATTGGATTGAAAGTGAAAAGAATGACACATGGAGGCTGAAAAGGGCCC10.9900990099009901No Hit
GAATGGCAGTCCTGGGGGATACAGCCTGGGACTTCGGATCAGTCGGGGGT10.9900990099009901No Hit
CATTCCATACCAGCAGCCGTCTTTTGCTCGAAACGATAGTGGGGGCATTG10.9900990099009901No Hit
GATGTCTTCTTAGGTTACTACAATCTTTATGACAATCAGTAAGAGAATAA10.9900990099009901No Hit
CCCTGACCCCAGTTGGAAGGCTGATAACCGCCAACCCCGTGATTAGTCAA10.9900990099009901No Hit
AAGCTTGTCCATTTTTAGGCGGCATTTCAAGTGGCCAGAGAACAGCTTTC10.9900990099009901No Hit
GTGCCACACTGAAGGAATGTCCGCTTGAGCACAGAGCATGGAATAGTTTT10.9900990099009901No Hit
CCTCTTTGTTGTTCCAGTGTGGAGTTCCGGTGTATGCCCCAGCATGCCAA10.9900990099009901No Hit
CTTGTGGAGGATCACGGGTTTGGAGTCTTCCACACCAGTGTCTGGCTTAA10.9900990099009901No Hit
ATGTAAGAATCCCCAAATGGTGGGTCAAGCTCCAACATCATCTTTGAGTT10.9900990099009901No Hit
GATGTGGGGTGCTCAGTGGACTTCTCAAAAAAGGAAACGAGATGTGGCAC10.9900990099009901No Hit
GTGACAGCGGGGTCAACCGATCATATGGACCACTTCTCTCTTGGAGTGCT10.9900990099009901No Hit
GAGTGTTTTCATGTCATCCCATTGCCTTGGCATGCTGGGGCAGACACCGG10.9900990099009901No Hit
ACAACATTGGGAGTGAACAAGTGCCACGTACAGATCATGGACCTCGGCCA10.9900990099009901No Hit
GTTACTAACCGTCGTCGTGACCAACGCTATGTCAACTGTTGGCTTGTACT10.9900990099009901No Hit
ATCTTTGAGTTCTCAGTGCTTTCAGTAATCACGGGGTTGGCGGTTATCAG10.9900990099009901No Hit
CTCCTATGCCAGTGGTGGGTGATTTTCTTGTCCCCAACTCCTATGACAAT10.9900990099009901No Hit
GTCGGCGGTGACACACTGAAGGAATGTCCGCTTGAGCACAGAGCATGGAA10.9900990099009901No Hit
GTCTTTAGCTGGTCCACTCAGCCACCACAACACCAGAGAGGGTTACAGAA10.9900990099009901No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.9900990099009901No Hit
GAAGATTACTCATTAGAATGTGACCCAGCCGTCATAGGAACAGCTGTTAA10.9900990099009901No Hit
TCTTCAGAGGAAGCTATCTGGCAGGAGCTTCCCTTATCTTTACAGTGACG10.9900990099009901No Hit
CTTATACACATCTCCGAGCCAACGAGACTAGGCATGATCTCGTATGACGT10.9900990099009901No Hit
CTCACATGCTTGGCCCTGGGGGGAGTGATGATATTCCTCTCCACGGCTGT10.9900990099009901No Hit
GTGGAGTTCCGGTGTCTGCCCAAGCATGCAAAGGCAATGGGATGTCATGA10.9900990099009901No Hit
TCCCAAAAGCCAGGCAATGGCAACGGCCACTAGCGCAAACCCGGGGTTCC10.9900990099009901No Hit
ATACTGTATGCCGGGGCAATCAGCAGTATCATGACCAAGTATATGACTTT10.9900990099009901No Hit
AGACACTGGTGTGGAAGACTCCAAACCCGTGATCCTCCACAAGAAAACCT10.9900990099009901No Hit
AGTTGGTGACAAGAAAATCACCCACCACTGGCATAGGAGTGGTAGCACCA10.9900990099009901No Hit
GGTAATACAGATCTGAAAAGTCAAGGCCTGTCCTTGGTTCACAGTCAAGT10.9900990099009901No Hit
TTTGAGTTCTCAGTGCTTTCAGTAATCACGGGGTTGGCGGTTATCAGCCT10.9900990099009901No Hit
GTCCTGGTTCTCACAGATCCTCATAGGCACGCTGCTAGTGGTGTCTCTTA10.9900990099009901No Hit
CGATAGTGGGGGCATTGTGCATTCCCTACAGCACCATTCCTCAATGACCC10.9900990099009901No Hit
GTCCAATTGGCTCTGAAAATGAAGGAGACCAGCCTTGCATGTCTCTTATA10.9900990099009901No Hit
GCACAAGGAATATGACACGCCCTTCAATCTAAGCTTGTGCATTTTTAGGC10.9900990099009901No Hit
GTAACCCTCTCTGGTGTTGTGGTGGCTGAGTGGACCAGCTAAAGACTTGG10.9900990099009901No Hit
GCCATTGATGTGCTCATGATGATCTTTGTGGTCATTCTCTTCTTCAACCC10.9900990099009901No Hit
GGCCAGAGAACAGCTTTCCCTTTGCACCATCCATCTCAGCCTCTAGAGCT10.9900990099009901No Hit
GTTGAGAATAATCAATGCTAGGAAAGAGAGGAAGAGACGTGGAGCAGACA10.9900990099009901No Hit
CGCTGGAGCTCTAGAGGCTGAGATGGATGGTGCAAAGGGAAAGCTGTTCT10.9900990099009901No Hit
TCCCAGGCCTGCTTGACTGCTGCTGCCAATCTGCGGGGGGAGTCAGGATG10.9900990099009901No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT10.9900990099009901TruSeq Adapter, Index 1 (95% over 21bp)
GTGATGATCTTCCTCTCCACGGCTGTTTCTGCTGATGTGGGGTGCTCAGT10.9900990099009901No Hit
GCCTATGAGGATCTGTGAGAACCAGGACATTCCTCCAAACAGTGATTTGA10.9900990099009901No Hit
CCCCAGCATGCCAAGGCAATGGGATGTCATGAAACCACTCTTTGTGCACC10.9900990099009901No Hit
ATTGTGGACAGGTGGAGTAGAAGAAAGTGATCTTATCATACCCATGTCTA10.9900990099009901No Hit
GACACCAGCATCGGAATCATTGGCCTCCTGCTGACTACAGCCATGGCAGC10.9900990099009901No Hit
CACATGTGTGACGCCACCATGAGTTATGAGTGCCCTATGCTGGATGAGGG10.9900990099009901No Hit
GATGTCATGAAACCACTCTTTGTGCACCAACCAATGCTTATTGTTCATGG10.9900990099009901No Hit
GTGCACTGCGGCATTCACATTCACCAAGGTCCCAGCTGAAACACTGCATG10.9900990099009901No Hit
AAAAAAAACAAAATTCAGTAAAAATGACAGATAATAATACATCTCTAAAA10.9900990099009901No Hit
GAAAGTAGCACCCATCAGGATCACAAGCTTGGCCAGGTCACTCATTGAAA10.9900990099009901No Hit
GGATCTGTGGGATCTCATCCGTTTCAAGAATGGAAAACATAATGTGGAAA10.9900990099009901No Hit
CTGAGGAAGTGTTAGAGATGCAAGACTTATGGTTGTTGAGGAAGCCAGAG10.9900990099009901No Hit
CTCATCCAGCATAGGGCACTCATAACTCATGGTGGCGTCACACATGTGCC10.9900990099009901No Hit
TACCAGAATAGCGTCATGATACTCAATGATACAGCATAAGAAACTGACGA10.9900990099009901No Hit
GTGTGACGCCACCATGAGTTATGAGTGCCCTATGCGGGATGAGGGAGTGG10.9900990099009901No Hit
GCTTGTGATTCTACTCATGGTGCAGGAGGGGTTGAAGAAGAGAATGACCA10.9900990099009901No Hit
CCCAAAAGCCAGGCAATGGCAACGGCCACTAGCGCAAACCCGGGGTTCCT10.9900990099009901No Hit
GACTACAGCCATGGCAGCAGAGATCACTAGACGCGGGAGTGCATACTACA10.9900990099009901No Hit
CTCCACGGCTGTTTCTGCTGATGTGGGGTGCTCAGTGGACTTCTCAAAAA10.9900990099009901No Hit
TGCTACCACTCCTATGCCAGTGGTGGGTGATTTTCTTGTCCCCAACTCCT10.9900990099009901No Hit
GTCGGGGGTGTGTTCAACTCACTGGGTAAGGGCATTCACCAGATTTTTGG10.9900990099009901No Hit
CCATCTGTCCCTGCATACTGCACCTCCACTGTGACTGTTCCATGCAGTGT10.9900990099009901No Hit
CTGTGGGATCTCATCCGTTTCAAGAATGGAAAACATCATGTGGAAATCAG10.9900990099009901No Hit
GGAGAATGGAGTTCAACTGACAGTTGTTGTGGGATCTGTAAAAAACCCCA10.9900990099009901No Hit
GTTCACTCCCAATGTGGTAGCAAACGAAATGGCCTTCCCGGCATCGCTCC10.9900990099009901No Hit
CTCATTCACAGGCACTGGCAATCTTTGTGGACCTCTCCACATGGGGTTTT10.9900990099009901No Hit
ATATGAAATGGAGATAAGGCCCAGGAAAGAACCAGAGAGCAACTTAGTGA10.9900990099009901No Hit
TTCTCACTCTACAAGGAAGTTGCAAACGCGGTCGCAGACCTGGTTAGAAT10.9900990099009901No Hit
ACATCTCGTTTCCTTTTTTGAGAAGTCCACTGAGCACCCCACATCAGCAG10.9900990099009901No Hit
GACTGGGAAGAGGGGAGCAATGGGATATCATCCCTTTCAAAAATGCAAAA10.9900990099009901No Hit
GCATAGGAGTGGTAGCACCATCGGAAAGGCATTTGAGGCCACTGTGAGAG10.9900990099009901No Hit
GCGGGGAGCCATGCACTGATAGCATTATCCGATACTCCAGATTTTACGGT10.9900990099009901No Hit
CTTGTGCACTGCGGCATTCACATTCACCAAGGTCCCAGCTGAAACACTGC10.9900990099009901No Hit
GTCCATCTGTCCCTGCATACTGCACCTCCACTGTGACTGTTCCATGCAGT10.9900990099009901No Hit
GACCTCGGGCACATGTGTGACGCCACCATGAGTTATGAGTGCCCTATGCT10.9900990099009901No Hit
GGAGACATGCGGAACTAGAGGACCATCTCTGAGATCAACTACTGCAAGTG10.9900990099009901No Hit
GACGAAAATAGAGCGAAAGTCGAGGTTACGCCTAATTCACCAAGCGCGGA10.9900990099009901No Hit
CCACGTAAACCTTGGTGCCTGGACATTCATCAAACCGGATTTCAAGCTCT10.9900990099009901No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG10.9900990099009901No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers