FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_mosquito_9_pcr2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_mosquito_9_pcr2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343284
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGAGATGGTGAGAGGCAGGAGTTACTCTAATGAACATATGTGTCC42231.230176763263071No Hit
GCACTGGAGATGGAGCGTGCACCACTTGGCTCTGCTCTGCTTCTTATGAA30700.8943032591090758No Hit
GCACTGGAGATGGCTGGCACAGGCCATCAGACTCCCTAGAAAGCAGCAGT27430.7990468533342655No Hit
GCACTGGAGATGGCTACTGCTATGAGTAACACTTGTCCCATCTTCTTCTC22790.6638818004917211No Hit
GCACTGGAGATGGGAAACAAGGACAGATATAAATACAGAAAGCATCTATT21550.627760105335524No Hit
GCACTGGAGATGGCGGTCAGGGACAGCCAGCCTTTTACCCTAGATGGTTC14130.411612542384731No Hit
GCACTGGAGATGGAGATGCCGAGGACGAATGAGTTGCAGATGCAGTTGGG13950.40636907050721854No Hit
GCACTGGAGATGGACCTGGCCCGCCTTTTGCTGCCACACAGCTTGATGGT12970.3778212791740949No Hit
GGAGATGGAGCGTGCACCACTTGGCTCTGCTCTGCTTCTTATGAAGACAT7950.2315866745901353No Hit
GCACTGGAGATGGGAAGCAAGACCCGGCTTCGTGTGGAAAGACCAGCTTC7760.22605189871942763No Hit
GCACTGGAGATGGAGGGCTAAAGGGTAGGGGATGGGCTTCTTAGCCACAG7250.21119539506647558No Hit
TCCCATGACAGCCCGCATGGGGACAGAGTAGGTTTGCTGGCTGCTTAGGG4850.1412824366996423No Hit
TACTACAACCCATCAGAGTCCCTAATTACAATCTCTACATCATGGATGAA4840.14099113270644714No Hit
GGTCTGAACAGACCCTAGCATCCATCCATCTCGGTTTGAGCACTCTCTTC4770.13895200475408118No Hit
GATATAAATACAGAAAGCATCTATTCTGTTTCATGAAAGTGAGATGAATG4580.13341722888337354No Hit
GAGATGGAGCGTGCACCACTTGGCTCTGCTCTGCTTCTTATGAAGACATG4140.12059985318278743No Hit
GTTCAGACCATGCGGCCCTGAAGTCGTTCAAAGAATTCGCCGCTGGAAAA4070.11856072523042147No Hit
GTCTACGACAATCCTTAGAGGGTACTCCCTGGAAAAGTACAGTGTATCCT3830.11156942939373814No Hit
TTTCTAACCTACACCTCATGAGGCTCTGGTGAGAGAAGGAATAAGGAGCA3810.11098682140734785No Hit
CCTCAAGACTGCTCCTTGGTGAATTATAAGCATATAAAGGAAAGCTTTGC3750.10923899744817703No Hit
CTCTAGGAGATGCCTAAAACCAGTCATACTTGATGGTGAGAGAGTCATCT3750.10923899744817703No Hit
GGAGATGGAGATGCCGAGGACGAATGAGTTGCAGATGCAGTTGGGCCTGA3640.1060346535230305No Hit
TCCTTAGAGGGTACTCCCTGGAAAAGTACAGTGTATCCTAAAAATATAAA3610.10516074154344507No Hit
TGCTAGTGCTGCTCAGAGGAGAGGACGTATAGGCAGGAACCCTAACAAAC3540.1031216135910791No Hit
CATTAATAGGGTCTACTACCCTCACAGCTGTCAATCCCAGGGCCATGACA3510.1022477016114937No Hit
GAACATATGTGTCCACTAAGCAAAGATGGCCGGGATACAGTGTCCGATGG3450.10049987765232285No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGAG41000.0111.85733
GCACTGG42000.0109.03851
CACTGGA45650.0100.305582
TGGAGAT46050.099.9033365
GATGGCG2400.098.99469
CTGGAGA47150.097.572624
GGAGATG51200.091.2606356
GATGGGA6250.087.547229
GAGATGG52050.087.142217
AGATGGG12900.077.5771568
AGATAAG2900.072.006551
GATGGCT15300.063.9965029
AGATGGA24200.056.2283368
AGATGGT15150.054.175268
GATGGAG18250.052.8628659
GATGGGG9150.044.0631689
AGATGGC32800.042.1440358
TAAGGCC5050.041.3442764
CTATAAA700.001091740641.1466031
GATGGAC6700.040.833599
Produced by FastQC (version 0.11.2)