FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_mosquito_4_-_101817_pcr1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_mosquito_4_-_101817_pcr1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682334
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGTGCGTGGCCGAGGTTGGGCGGCGGTGCCTAGCCAAGTTCGCGGAA45180.6621390697224527No Hit
GTGCGTGGCCGAGCAGGTGCGCGAGGTGGAGGAGTGCCTGGCCTTCTGCG39000.5715675900658621No Hit
GTCAGTGCGTGGCCGAGCAGGTGCGCGAGGTGGAGGAGTGCCTGGCCTTC35890.5259887386529178No Hit
GTGCGTGGCCGAGGTTGGGCGGCGGTGCCTAGCCAAGTTCGCGGAACAGC32410.47498732292396395No Hit
GTCTTAACATAGGCAACATGAGCTCTAAGCTGAGCAGATGCAGTATCGCG18760.27493866640091214No Hit
GTGCGTGGCGGTTCTGATTTCGGTGAATCGCGCGACAACCGTGTTGCGAG16460.24123083416625876No Hit
GTATTACCTGACCATGAACAATAAGCATTGGTTGGTGCACAAAGAGTGGT13370.1959450943379635No Hit
CCTTAAACCTGTCTTGTAACCTTGATACTTACTTTCAGCCTCCATGTGTC13120.192281199529849No Hit
ATCTAAGCTTGTCCATTTTTAGGCGGCATTTCAAGTGGCCAGAGAACAGC12220.1790911782206368No Hit
TCCCAGGCGCGTGGCGTCGTCCGCCGTGGCCAGGTAGGGGTCGTCACCGT12030.1763066181664698No Hit
ATGCTGGATGAGGGAGTGGAACCAGATGATGTCGATTGCTGGTGCAACAC11780.1726427233583553No Hit
GGTAATACAGATCTGAAAAGTCAAGGCCTGTCCTTGGTTCACAGTCAAGT11680.1711771654351095No Hit
GGTTTAAGGAGACCAATAGAAACTGGGCTTGTCGAGACAGAGAAGACTCT11510.16868571696559162No Hit
GTCATAGGAGTTGGGGACAAGAAAATCACCCACCACTGGCATAGGAGTGG11350.16634082428839833No Hit
GTCAGTGCGTGGCTCCAGGTGCGGATGTCTGAAGGACCACTCAGGAACCT11040.16179759472633637No Hit
CTACATGTACTTGGATAGGAGCGATGCCGGGAAGGCCATTTCGTTTGCTA10970.1607717041800643No Hit
GTGCGTGGCGGCTGGTAGGAAGTAGTAAGTAAGAAAAGAAGAATACAAGT10770.1578405883335727No Hit
GTCAGTGCGTGGCGGTTCTGATTTCGGTGAATCGCGCGACAACCGTGTTG10400.15241802401756324No Hit
AGTCTGAAGCGAGAGCTAACAACAGTATCAACAGGTTTAATTTGGATTTG10300.15095246609431745No Hit
GTATATGACTTTTTGGCTCGTCGAGCTTCCCAAAAGCCAGGCAATGGCAA10160.14890068500177334No Hit
GTGGAGGAGTGCCTGGCCTTCTGCGGCCAGCGGCCGGTGCGCTGGCTGCT10050.14728857128620293No Hit
AACTAGATGTTGGTAACACGAACAATAGCGACATTAGCATTCTTAGGTTA9920.1453833459859834No Hit
GGCCGAGGTTGGGCGGCGGTGCCTAGCCAAGTTCGCGGAACAGCCGGGTC9800.14362467647808844No Hit
GTGCGTGGCTCCAGGTGCGGATGTCTGAAGGACCACTCAGGAACCTTAGT9780.1433315648934393No Hit
TCTTAGAACACGTAAACTTGGCACATGTCACCAAGCTCCCTTTGCCAAAA9650.14142633959321974No Hit
TTCATATCCTGTATCATTGACAATCATCCCGCTATGCTGGGAGCCATGCA9650.14142633959321974No Hit
TCCTTGTAGAGTGAGAAGGGAGCGTCACGGCTCTTCTAGATCGCCGTGCC9140.13395199418466616No Hit
GAATATGACACGCCCTTCAATCTAAGCTTGTCCATTTTTAGGCGGCATTT8860.1298484319995779No Hit
GTGCGTGGCAAACTAGATGTTGGTAACACGAACAATAGCGACATTAGCAT8760.12838287407633212No Hit
GCCGAGCAGGTGCGCGAGGTGGAGGAGTGCCTGGCCTTCTGCGGCCAGCG8700.12750353932238465No Hit
CTCCAAGAGAGAAGTGGTCCATATGATCGGTTGACCCCGCTGTCACCATT8700.12750353932238465No Hit
TCCTAGAGGAGAATGGAGTTCAACTGACAGTTGTTGTGGGATCTGTAAAA8660.1269173161530863No Hit
TAATAAAGAAGTTCAAGAAAGATCTTGCTGCCATGTTGAGAATAATCAAT8560.1254517582298405No Hit
AGGTTGGGCGGCGGTGCCTAGCCAAGTTCGCGGAACAGCCGGGTCAGCGC8440.12369308872194555No Hit
GTCTTTAGCTGGTCCACTCAGCCACCACAACACCAGAGAGGGTTACAGAA8430.12354653292962096No Hit
GTCAGTGCGTGGCGGCTGGTAGGAAGTAGTAAGTAAGAAAAGAAGAATAC8430.12354653292962096No Hit
TCCGTACACAACCCAAGTTGATGTCGTGTTGCACCAGCAATCGACATCAT8230.12061541708312938No Hit
CTCAATATGTCTGCAAAAGAACATTAGTGGACAGAGGTTGGGGAAACGGT8170.1197360823291819No Hit
GGTAGGGGTCGTCACCGTCGAGCACCACCAGGTCGGCACGCAGGCCCGGG7990.11709807806733945No Hit
TGTCAGTGCGTGGCCGAGGTTGGGCGGCGGTGCCTAGCCAAGTTCGCGGA7910.11592563172874282No Hit
GAGTTATGAGTGCCCTATGCTGGATGAGGGAGTGGAACCAGATGATGTCG7780.11402040642852326No Hit
GTTAGTAACATGGCCGAGGTAAGATCCTATTGCTACGAGGCATCGATATC7780.11402040642852326No Hit
AGGTGGAGGAGTGCCTGGCCTTCTGCGGCCAGCGGCCGGTGCGCTGGCTG7680.11255484850527747No Hit
CTACAAGGAAGTTGCAAACGCGGTCGCAGACCTGGTTAGAATCAAGAGAA7640.11196862533597916No Hit
ATCTAGAAGAGCCGTGACGCTCCCTTCTCACTCTACAAGGAAGTTGCAAA7570.1109427347897071No Hit
AGCTTAGATTGAAGGGCGTGTCATATTCCTTGTGCACTGCGGCATTCACA7550.11064962320505793No Hit
TTAGTGGACAGAGGTTGGGGAAACGGTTGTGGACTTTTTGGCAAAGGGAG7530.11035651162040877No Hit
TCCATGCTCTGTGCTCAAGCGGACATTCCTTCAGTGTGTCACCGTCGACA7520.1102099558280842No Hit
TCCCTTAACAGCTGTTCCTATGACGGCTGGGTCACATTCTAATGAGTAAT7500.10991684424343504No Hit
GTGCGTGGCACGGCCATTGCTCGAATTGCCAACCAGGCCAAAGCAAATCC7360.10786506315089092No Hit
CTTCTAGATCGCCGTGCCTCACCTTTTTTGTGATGACAGGTTCCGTACAC7350.10771850735856635No Hit
GTACAAGTACCATCCTGACTCCCCCCGCAGATTGGCAGCAGCAGTCAAGC7320.10727883998159259No Hit
GTAGTATGCACTCCCGCGTCTAGTGATCTCTGCTGCCATGGCTGTAGTCA7240.10610639364299596No Hit
TCTCTCCACAGAGGGCCCACCTGATTGAGATGAAAACATGTGAATGGCCA7230.10595983785067137No Hit
CATATTGAGTGTCTGATTGCTTGTCAAGGTAGGCCTCACCTTGTGTTGGG7210.10566672626602222No Hit
TTCTTACATTGTCATAGGAGTTGGGGACAAGAAAATCACCCACCACTGGC7150.10478739151207474No Hit
GTACAGATCATGGACCTCGGGCACATGTGTGACGCCACCATGAGTTATGA7070.10361494517347809No Hit
GGATAGGAGCGATGCCGGGAAGGCCATTTCGTTTGCTACCACATTGGGAG7070.10361494517347809No Hit
ACTCTATGTCAACTGTTGGCTTGTCCTGTGCCATCACGGTAACGCAGCCT6920.10141660828860939No Hit
TTATAGATGAATACCCCCGTGCCACATCTCGTTTCCTTTTTTGAGAAGTC6900.10112349670396022No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAGTG18450.0107.339361
CAGTGCG18800.0106.466783
AGTGCGT18800.0106.083814
GTTCGAG1700.097.4321
CGAGTCT1750.094.627424
TCGAGTC1750.094.627423
CTGACAA1250.092.1588759
TTGTCAG406.3646803E-790.018691
TTCGAGT1850.089.512422
CGACAGT353.55283E-582.30281
ACAGTTC406.894432E-571.999123
GTGGCTC4300.070.324729
CGGTTCT4600.067.303539
GAGTCTG2500.066.239195
GCGGTTC4800.065.999198
AGTCTGA3100.062.7227021
TGCGTGG34300.062.1333542
GTGCGTG34200.061.6970251
CTGAAGC3350.058.0291444
GCCGAGC10600.057.735148
Produced by FastQC (version 0.11.2)