FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_mosquito_1_-_101817_pcr3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_mosquito_1_-_101817_pcr3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720051
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTAATGGGAGTGAAGGAAAATTTCATAGGCTTGTTGCAAATAGAAAGAA97531.354487390476508No Hit
CACAACACACCTAGGATGGTTTTGCACAAAATTGATTATCGCAATTATAC33050.45899526561313014No Hit
CACCTAGGATGGTTTTGCACAAAATTGATTATCGCAATTATACCTTTTCC31300.434691431579152No Hit
CATCTAATGGGAGTGAAGGAAAATTTCATAGGCTTGTTGCAAATAGAAAG30280.42052576831363336No Hit
CACACACCTAGGATGGTTTTGCACAAAATTGATTATCGCAATTATACCTT25960.360530018012613No Hit
GGTGTGGACAGTGATGAGTGAGGAATAAAGAATAACAAATGAGACGTGAG18760.26053710084424575No Hit
GTATAGATAAGGGAAGCTCCTGCCAGATAGCTTCCTCTGAAGATGTTGCA17980.24970453481767263No Hit
TAATGGGAGTGAAGGAAAATTTCATAGGCTTGTTGCAAATAGAAAGAAAA17850.24789910714657712No Hit
TCCCCACACACACCTAGGATGGTTTTGCACAAAATTGATTATCGCAATTA17380.2413717917203087No Hit
TATCTATACAGTGACGAGAAACGCTGGCCTGGTTAAGAGACGTGGAGGTG15010.20845745648572114No Hit
GTGATGAGTGAGGAATAAAGAATAACAAATGAGACGTGAGGAATGAGGAA14370.19956919718186628No Hit
ACACACACCTAGGATGGTTTTGCACAAAATTGATTATCGCAATTATACCT13110.18207043667740203No Hit
GTGTTTACAACTAGTGCAAATGCCGAAAGTACCGATATATCATCAGTGCG12960.17998725090306103No Hit
GTTTACAACTAGTGCAAATGCCGAAAGTACCGATATATCATCAGTGCGTA11970.16623822479241052No Hit
GTGTGTACAACTAGTGCAAATGCCGAAAGTACCGATATATCATCAGTGCG11820.16415503901806955No Hit
AATGGGAGTGAAGGAAAATTTCATAGGCTTGTTGCAAATAGAAAGAAAAC11080.15387798919798737No Hit
TTGTACACACTTGCTTTCCACTTTACTGCTCTGCTTGCTTTTTTGTGTTG11070.15373911014636463No Hit
GGCTTGTTGCAAATAGAAAGAAAACGGCAGCTGCCAAGAATTCGCAACAC10930.15179480342364637No Hit
CCTAGGATGGTTTTGCACAAAATTGATTATCGCAATTATACCTTTTCCCC9750.13540707533216395No Hit
GGTGTGGACAGTGATGAGTGAGGAATAAAGAATAACAAATGAGGCGTGAG9210.12790760654453642No Hit
TCCCCACACTTACTCACTCTTCGCTTTTATTATTGAATCGTGGATTGCTC9200.1277687274929137No Hit
TTTCATAGGCTTGTTGCAAATAGAAAGAAAACGGCAGCTGCCAAGAATTC8940.12415787215072265No Hit
GGACAGTGATGAGTGAGGAATAAAGAATAACAAATGAGACGTGAGGAATG8810.12235244447962713No Hit
TTGTAAACACTTGCTTTCCACTTTACTGCTCTGCTTGCTTTTTTGTGTTG8360.11610288715660419No Hit
ACATGTACTTGATCCCAGGCCTACAAGCGGCAGCAGCGCGTGCTGCCCAG8110.11263091086603587No Hit
CACCACACCTAGGATGGTTTTGCACAAAATTGATTATCGCAATTATACCT8110.11263091086603587No Hit
GTTGCAAATAGAAAGAAAACGGCAGCTGCCAAGAATTCGCAACACAAAAA8060.11193651560792223No Hit
GTATAAAGGTGCTGTGTCCATACACCAGCACTATGATGGAAACCATGGAG8010.11124212034980856No Hit
ATCTAATGGGAGTGAAGGAAAATTTCATAGGCTTGTTGCAAATAGAAAGA7910.10985332983358123No Hit
TGGTTGAGGTTGCCGGAGGAAGCAGACGAGAAATATTTCAAGTGCAGTGA7850.10902005552384485No Hit
CCTTTATACAGAAGGCCCCTGGTCTTTTTTCAAGCCAGTCCCCCACCATA7800.10832566026573118No Hit
CACTTACTCACTCTTCGCTTTTATTATTGAATCGTGGATTGCTCAACGAT7650.1062424744913902No Hit
ATTATACCTTTTCCCCAAAACAGATTACGCACTGATGATATATCGGTACT7530.1045759258719174No Hit
AAATTGATTATCGCAATTATACCTTTTCCCCAAAACAGATTACGCACTGA7360.10221498199433096No Hit
ATACAGAACAGGATACACGTGTGTGCGCGTGCGATCATTCGTTTACAAAA7300.10138170768459456No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCCAC13750.0101.6108861
CATCTAA7250.087.4149861
CCCCACA16950.082.829582
TGGGAGT19350.078.137396
TAATGGG22500.074.237963
CCCACAC19400.072.7402653
GGGTGTG4450.069.5904541
CTAATGG25450.065.6327742
AACACAC8700.064.549944
TGTGGAC14750.062.9677623
CACAACA8900.061.498541
GGGAGTG25000.061.342317
AATGGGA27350.061.336524
ACAACAC10300.054.5227782
GGACAGG400.00621196454.013521
ATGGGAG28600.053.369165
ACAGGTC953.3143806E-853.0511748
GAGTGAA29300.052.8313149
TCTAATG32550.051.7733271
GAGTGCG3150.050.2843328
Produced by FastQC (version 0.11.2)