FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_mosquito_19_f4_pcr2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_mosquito_19_f4_pcr2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341187
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGAGATGGCTACTGCTATGAGTAACACTTGTCCCATCTTCTTCTC13390.39245340531731865No Hit
CAGTATGACACCAAGTCCTGCTTGACATCCCCCCAGTATGGATCAAGTCT5610.1644259599574427No Hit
GGTCTGAACAGACCCTAGCATCCATCCATCTCGGTTTGAGCACTCTCTTC4990.1462541069853189No Hit
AGATAAGGCCCAGGAAAGAACCAGAGAGCAACTTAGTGAGGTCAATGGTG4690.1374612749020332No Hit
CTATTAATGTGGTAGGACTACTGTTACTCACAAGGAGTGGGAAGCGGAGC4680.137168180499257No Hit
CATTAATAGGGTCTACTACCCTCACAGCTGTCAATCCCAGGGCCATGACA4590.1345303308742713No Hit
TACTACAACCCATCAGAGTCCCTAATTACAATCTCTACATCATGGATGAA4460.13072010363818082No Hit
CCCCGTGAGAGCATGCTGCTAGCCCTGGCTTCGTGTCTTCTGCAAACTGC4340.12720297080486653No Hit
GGATGAGAGTGGTGATTTCTCCTTGGTAGAGGAAGATGGTCCACCCATGA3850.11284134506883321No Hit
TACACATACCACGCTCCTGCAGCAAAAGGTATAGCTATTGGGTTCATGCC3830.11225515626328085No Hit
TGCTAGTGCTGCTCAGAGGAGAGGACGTATAGGCAGGAACCCTAACAAAC3710.10873802342996657No Hit
GTTCAGACCATGCGGCCCTGAAGTCGTTCAAAGAATTCGCCGCTGGAAAA3620.10610017380498085No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTGC7900.0102.075222
CTGTGCC9500.085.6414953
TAGAGTT353.5540645E-582.2851265
TGTGCCT12050.066.9206854
CTGGAGA8050.061.713854
ACTGGAG7850.061.4517943
CCTGTTC753.9378574E-757.599594
TGGAGAT9750.051.6919365
GATGGGG701.7644768E-551.4282049
TGCCTGA4300.050.2321972
TCTTAGA450.00990101247.9996571
AGATAAG2750.044.5087741
ACAGGCG1307.788913E-944.3073778
CCTGACA657.584167E-444.3073772
GCCTGTT1851.8189894E-1242.81053
GGCTTAC855.5565808E-542.3526421
GAGTTAT855.5565808E-542.3526429
GTGCCTG21300.041.5771681
ATCTGTG19950.040.0598641
CCTGAGA1452.0425432E-839.7238544
Produced by FastQC (version 0.11.2)