FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_mosquito_19_e8_pcr2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_mosquito_19_e8_pcr2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538952
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGAGATGGTGAGAGGCAGGAGTTACTCTAATGAACATATGTGTCC95981.7808636019534207No Hit
GCACTGGAGATGGAGCGTGCACCACTTGGCTCTGCTCTGCTTCTTATGAA68871.2778503465985838No Hit
GCACTGGAGATGGCGGTCAGGGACAGCCAGCCTTTTACCCTAGATGGTTC42750.7932060740103015No Hit
GCACTGGAGATGGCTGGCACAGGCCATCAGACTCCCTAGAAAGCAGCAGT35760.6635099229615996No Hit
GCACTGGAGATGGGAAACAAGGACAGATATAAATACAGAAAGCATCTATT28200.5232376909260936No Hit
GCACTGGAGATGGCTACTGCTATGAGTAACACTTGTCCCATCTTCTTCTC27280.5061675251228309No Hit
GCACTGGAGATGGAGATGCCGAGGACGAATGAGTTGCAGATGCAGTTGGG25290.46924401430925206No Hit
GCACTGGAGATGGACCTGGCCCGCCTTTTGCTGCCACACAGCTTGATGGT24350.4518027579450489No Hit
GGAGATGGAGCGTGCACCACTTGGCTCTGCTCTGCTTCTTATGAAGACAT18270.33899122741913934No Hit
GCACTGGAGATGGGAAGCAAGACCCGGCTTCGTGTGGAAAGACCAGCTTC14120.2619899360239873No Hit
GCACTGGAGATGGCTCAGCGGTAAAGACTCTTGCCAGGAAAAGCCTGAGT11540.21411925366266382No Hit
TTTCTAACCTACACCTCATGAGGCTCTGGTGAGAGAAGGAATAAGGAGCA10730.19909008594457392No Hit
GTCTTGAGGCATCGAGGATGCTGATTGTCTGTGTCTGTGCACAGCATCTG10290.1909260936038831No Hit
GCACTGGAGATGGTGGAGGCAGAGGGATCAGGCATTCATAACTTCAGCTA10270.1905550030429426No Hit
GAGATGGAGCGTGCACCACTTGGCTCTGCTCTGCTTCTTATGAAGACATG9730.18053555789754933No Hit
GTCTACGACAATCCTTAGAGGGTACTCCCTGGAAAAGTACAGTGTATCCT8570.1590123053630008No Hit
GGTTAGAAATGACCCAACAGCCAGCAGGTGGCAAAGGCTACCATCAGCCT8430.15641467143641735No Hit
GAACATATGTGTCCACTAAGCAAAGATGGCCGGGATACAGTGTCCGATGG8100.15029167718089922No Hit
CCTCAAGACTGCTCCTTGGTGAATTATAAGCATATAAAGGAAAGCTTTGC8080.14992058661995872No Hit
TCCTTAGAGGGTACTCCCTGGAAAAGTACAGTGTATCCTAAAAATATAAA8050.149363950778548No Hit
GCACTGGAGATGGCTGAGCCGTCTTTCTAGCCATCTTTCTACTGTAAGAT7990.1482506790957265No Hit
GGAGATGGAGATGCCGAGGACGAATGAGTTGCAGATGCAGTTGGGCCTGA7700.1428698659620894No Hit
GCACTGGAGATGGCGGAAGGGAGAGAAGATGGGTCCCTGGATTTTACTGA7300.13544805474327953No Hit
GTTTAGGAGAGGATGGAAATTTGTTTCACACCTGGCTGCTCCTTATTCCT6910.12821178880493997No Hit
CTCCTAAACCAAGCTCTTTCCTGGGAAAGATGGCCAAGGCGAGACCTAGA6840.12691297184164824No Hit
CCTTATTCCTTCTCTCACCAGAGCCTCATGAGGTGTAGGTTAGAAATGAC6720.12468642847600529No Hit
TCCCATGACAGCCCGCATGGGGACAGAGTAGGTTTGCTGGCTGCTTAGGG6660.12357315679318381No Hit
GCACTGGAGATGGTGAGAGATGTACTCAGAACATCCCAGTAAACTCTTTC6580.12208879454942184No Hit
GGTCTGAACAGACCCTAGCATCCATCCATCTCGGTTTGAGCACTCTCTTC6560.12171770398848133No Hit
GGAGATGGTGAGAGGCAGGAGTTACTCTAATGAACATATGTGTCCACTAA6430.11930561534236817No Hit
GCACTGGAGATGGTTCAGTGGGTAGAGGCTCTCATCACCAAACCTGACAG6180.114666983330612No Hit
TACTACAACCCATCAGAGTCCCTAATTACAATCTCTACATCATGGATGAA5880.10910062491650463No Hit
GTATAGGTAAACACTACACCCTGTCTGGGGAATGCTGAAGTTAGATTTAG5770.10705962683133191No Hit
ACTGGAGATGGAGCGTGCACCACTTGGCTCTGCTCTGCTTCTTATGAAGA5640.10464753818521873No Hit
GCTTATAATTCACCAAGGAGCAGTCTTGAGGCATCGAGGATGCTGATTGT5520.10242099481957577No Hit
ATCTAGGTCTCGCCTTGGCCATCTTTCCCAGGAAAGAGCTTGGTTTAGGA5420.10056554201487332No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGAG69450.0119.325523
GCACTGG70450.0117.346931
CACTGGA73800.0112.779912
GATGGCG7900.0109.356389
TGGAGAT78250.0106.274225
CTGGAGA79300.0104.594674
GGAGATG85850.097.95646
ACGGGAG301.6547232E-595.999513
GAGATGG89000.092.94377
GCACGGG353.552632E-582.300561
CACGGGA353.555904E-582.2852862
AGATAAG6250.081.806761
GATGGGA10450.075.782059
GATGGCT24750.072.720159
AGATGGG18450.067.115388
AGATGGT32300.065.083418
AGATGGA36400.058.5437168
TAAGGCC9850.052.629174
GATGGAC8850.050.4357389
GCGTCAT450.00989676848.008661
Produced by FastQC (version 0.11.2)