FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_mosquito_18_pcr1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_mosquito_18_pcr1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences828578
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTAACATAGGCAACATGAGCTCTAAGCTGAGCAGATGCAGTATCGCG54390.6564258283468787No Hit
GTCAGTGCGTGGCTCCAGGTGCGGATGTCTGAAGGACCACTCAGGAACCT26600.3210319366432611No Hit
GTCTTAGGTATGGGTGTTGTCTTACTGCGTTCTTTGGAGGCATGATTTTG24990.3016010562674848No Hit
GTGCGTGGCTCCAGGTGCGGATGTCTGAAGGACCACTCAGGAACCTTAGT24560.29641144225407867No Hit
AACTAGATGTTGGTAACACGAACAATAGCGACATTAGCATTCTTAGGTTA23490.2834977515695565No Hit
GTGCGTGGCAAACTAGATGTTGGTAACACGAACAATAGCGACATTAGCAT20500.2474118308716862No Hit
TTCTTAGGTTAATGATTCTACTGTCTTAACATAGGCAACATGAGCTCTAA17620.21265348585166396No Hit
GGACTAGATCGCGCATGAGCTTGGTGTGTTTATTTTGAATTTCATTGGCA16260.1962398229255423No Hit
GGCTCCAGGTGCGGATGTCTGAAGGACCACTCAGGAACCTTAGTGCAAGC16230.19587775683158376No Hit
CTTAAGAACTGTGGATATTTTGCAGATCTAATTAAGAAAAATGTTTTGAC14900.1798261599994207No Hit
ATCATAATCTCTGCGATTAACCATCACCTTGTTAAACGCTCATACCTTCA14560.1757227442678903No Hit
ATCTAAGCTTGTCCATTTTTAGGCGGCATTTCAAGTGGCCAGAGAACAGC14490.17487792338198696No Hit
CCTTAAACCTGTCTTGTAACCTTGATACTTACTTTCAGCCTCCATGTGTC14300.17258483812024938No Hit
GTTCTTAAGCGTGTGATTCAAAATACACAAGTTAAAGATTATTTTTCAAT13790.1664297145229538No Hit
GTGCGTGGCTTGGGGTGGGGCTTACCGCTCGGAGCTGCCGGTCCGAGGTG13190.15918839264378248No Hit
CTCAGGAACCTTAGTGCAAGCTTATCTTGGAACACTTTTAGCAATTCCAT13010.15701599608003108No Hit
GTCAGTGCGTGGCAAACTAGATGTTGGTAACACGAACAATAGCGACATTA12940.15617117519412776No Hit
GGTAATACAGATCTGAAAAGTCAAGGCCTGTCCTTGGTTCACAGTCAAGT12750.1538780899323902No Hit
GGTTTAAGGAGACCAATAGAAACTGGGCTTGTCGAGACAGAGAAGACTCT12700.15327464644245925No Hit
GTATTACCTGACCATGAACAATAAGCATTGGTTGGTGCACAAAGAGTGGT12490.1507401837847493No Hit
CCTTAGTGCAAGCTTATCTTGGAACACTTTTAGCAATTCCATGTCTTGCT12060.1455505697713432No Hit
GGCTTGGGGTGGGGCTTACCGCTCGGAGCTGCCGGTCCGAGGTGCCCAGA11920.14386092799953656No Hit
ATGCTGGATGAGGGAGTGGAACCAGATGATGTCGATTGCTGGTGCAACAC11860.14313679581161942No Hit
GTCATAGGAGTTGGGGACAAGAAAATCACCCACCACTGGCATAGGAGTGG11860.14313679581161942No Hit
GTCTTTAGCTGGTCCACTCAGCCACCACAACACCAGAGAGGGTTACAGAA11210.13529203044251717No Hit
CTGAAGGACCACTCAGGAACCTTAGTGCAAGCTTATCTTGGAACACTTTT10970.13239550169084868No Hit
TGTCTGAAGGACCACTCAGGAACCTTAGTGCAAGCTTATCTTGGAACACT10900.13155068080494534No Hit
GCGTGGCTCCAGGTGCGGATGTCTGAAGGACCACTCAGGAACCTTAGTGC10790.13022310512709728No Hit
AGTCTGAAGCGAGAGCTAACAACAGTATCAACAGGTTTAATTTGGATTTG10700.12913690684522158No Hit
TAATAAAGAAGTTCAAGAAAGATCTTGCTGCCATGTTGAGAATAATCAAT10620.12817139726133206No Hit
ATCTAGTCCATGAGGTCGTCGTACATGCCCGGTACGAGAAGCAAGACATG10580.1276886424693873No Hit
GTCAGTGCGTGGCGCAATTTGACTGCCGTCTTGCGCATGCCCTTCTACAC10510.126843821583484No Hit
AGCTTATCTTGGAACACTTTTAGCAATTCCATGTCTTGCTTCTCGTACCG10420.1257576233016083No Hit
TTCATATCCTGTATCATTGACAATCATCCCGCTATGCTGGGAGCCATGCA10320.12455073632174642No Hit
GTCTGAAGGACCACTCAGGAACCTTAGTGCAAGCTTATCTTGGAACACTT10280.12406798152980165No Hit
GCTTGGGGTGGGGCTTACCGCTCGGAGCTGCCGGTCCGAGGTGCCCAGAG10240.1235852267378569No Hit
CTTTTAGCAATTCCATGTCTTGCTTCTCGTACCGGGCATGTACGACGACC10110.12201627366403645No Hit
GATTATGATTGAAAAATAATCTTTAACTTGTGTATTTTGAATCACACGCT9960.12020594319424363No Hit
CTACATGTACTTGGATAGGAGCGATGCCGGGAAGGCCATTTCGTTTGCTA9960.12020594319424363No Hit
GTATATGACTTTTTGGCTCGTCGAGCTTCCCAAAAGCCAGGCAATGGCAA9930.11984387710028506No Hit
TCTTAGAACACGTAAACTTGGCACATGTCACCAAGCTCCCTTTGCCAAAA9890.11936112230834031No Hit
TCCGTACACAACCCAAGTTGATGTCGTGTTGCACCAGCAATCGACATCAT9870.11911974491236794No Hit
CTCCAAGAGAGAAGTGGTCCATATGATCGGTTGACCCCGCTGTCACCATT9480.1144128856909066No Hit
ATTCCATGTCTTGCTTCTCGTACCGGGCATGTACGACGACCTCATGGACT9420.11368875350298946No Hit
TCCTTGTAGAGTGAGAAGGGAGCGTCACGGCTCTTCTAGATCGCCGTGCC9220.11127497954326569No Hit
TTAGTGGACAGAGGTTGGGGAAACGGTTGTGGACTTTTTGGCAAAGGGAG9180.11079222475132094No Hit
GTAGTATGCACTCCCGCGTCTAGTGATCTCTGCTGCCATGGCTGTAGTCA9160.11055084735534855No Hit
TGTCAGTGCGTGGCAAACTAGATGTTGGTAACACGAACAATAGCGACATT9030.10898189428152812No Hit
GCTCCAGGTGCGGATGTCTGAAGGACCACTCAGGAACCTTAGTGCAAGCT8880.10717156381173527No Hit
GGCATGTACGACGACCTCATGGACTAGATCGCGCATGAGCTTGGTGTGTT8870.1070508751137491No Hit
TCTCCATTCCATACCAGCAGCCGTCTTTTGCTCGAAACGATAGTGGGGGC8830.10656812032180435No Hit
ATTCTACTGTCTTAACATAGGCAACATGAGCTCTAAGCTGAGCAGATGCA8810.10632674292583198No Hit
GGATAGGAGCGATGCCGGGAAGGCCATTTCGTTTGCTACCACATTGGGAG8680.10475778985201151No Hit
CATATTGAGTGTCTGATTGCTTGTCAAGGTAGGCCTCACCTTGTGTTGGG8660.10451641245603914No Hit
GAATATGACACGCCCTTCAATCTAAGCTTGTCCATTTTTAGGCGGCATTT8610.10391296896610819No Hit
TTATAGATGAATACCCCCGTGCCACATCTCGTTTCCTTTTTTGAGAAGTC8540.10306814808020488No Hit
TCCTAGAGGAGAATGGAGTTCAACTGACAGTTGTTGTGGGATCTGTAAAA8480.10234401589228775No Hit
CTTCTAGATCGCCGTGCCTCACCTTTTTTGTGATGACAGGTTCCGTACAC8470.10222332719430156No Hit
ATCTAGTTGTAGCATAAAGGGGACGATATGTTGTTGCGTAGGGTAGACTC8300.10017161932853635No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGAAA450.0128.004562
GTTCGAG1350.0101.336951
GTCAGTG11300.094.941441
CAGTGCG11400.094.108623
TCGAGTC1700.093.179793
GTTCGAA554.4019544E-1091.6396261
AGTGCGT11800.089.698114
CGAAACC651.6370905E-1188.6185464
GAACCGA703.274181E-1182.288646
AGAACCG851.8007995E-1067.767125
AACCGAG903.0013325E-1063.998427
GAGTCTG2550.062.119865
AGTCTGA2900.062.071181
ATCTACA350.003664889161.7164842
GGCAGAA954.8567017E-1060.6337362
GTGCGTG19250.058.3501321
TGCGTGG19450.058.120322
CGTGGCT9650.057.4528274
CTGAAGC3150.057.1448944
GTGGCTT3050.056.6577575
Produced by FastQC (version 0.11.2)