FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_lp_negative_control_cb10-14_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_lp_negative_control_cb10-14_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length150
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGCGGATTCTGAAAGAAGAATCCGATGAGGCACTTAAAATGACCATGG23.4482758620689653No Hit
GCTAATGATGGGTTGCTATTCACAATTAACACCCCTGACTCTGATAGTAG11.7241379310344827No Hit
ACTCCAAGAGTGTTCATTTCTGCGAAAGAAGCACCCATCAGTATCACAAG11.7241379310344827No Hit
TAATTGCCATCTGGTTATCTTGGGGAGATCTTTGCTTCTCTGGCTCGGGT11.7241379310344827No Hit
AGCTATACCTTTTGCTGCAGGAGCGTGGTATGTGTATGTGAAGACTGGGA11.7241379310344827No Hit
ACTTAACCCGAATATCAAATGGGAATCAAACATTATGTAGAGATTCTAAA11.7241379310344827No Hit
ACTACAGCTAGAGTCGCATTCGCCAAAAAGGTGTTGTTGATGTAACTAAA11.7241379310344827No Hit
AATCATCGGCAGGCGCATTGAGAGAATCCGCAATGAACATGCAGAAACAT11.7241379310344827No Hit
GTCCAAAGCAAATCGAATAATGAGGAAAATCAATTCACCTTCAACAGCAG11.7241379310344827No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT11.7241379310344827No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT11.7241379310344827No Hit
CTACATCATGGATTAAGCCCACTTCCCATACCCCGCAAGGAGAGCTGCAA11.7241379310344827No Hit
TTTTTCTCCTTCAGCTGGTCTGAGAACCTTCACCACTTTGTTTTGGTATG11.7241379310344827No Hit
GGCCTACTGTTTCTCAGAAGGAGTGTGAAGCGGAGATGGCCCACTAGACA11.7241379310344827No Hit
TTATCGGCCTCAGGCCGATAGAGCGAGGCTTTCAGGCCATCCTGGAGCTA11.7241379310344827No Hit
GGCCATGGCCACACAAGCTGGAGTGCTGTTTGGCATGGGCAAAGGGATGC11.7241379310344827No Hit
GTGCAAGATGGCGCCCTTCATTGCAGCTGTCTTAGGCTCCATATCGCGAG11.7241379310344827No Hit
GACAGAAAGTGGATGAGAGAACTTATCCTTTATGACAAAGAAGAAATAAG11.7241379310344827No Hit
GAGAGAAGAAGGAGCAACCATGGGATTCTCAATGGACATTGATCTGCGGC11.7241379310344827No Hit
CCATCAAAAAGTCAAATTACCTACCCCTGGTGACTAACGAGAACAAAACA11.7241379310344827No Hit
CCCAAACACCATTTAACACATCCTCATAAGAATCTCCTGGAAGACAACGA11.7241379310344827No Hit
ATGGGAAGGAGAGAAGAAGGAGCAAACATGGGATTCTCAATGGACATTGA11.7241379310344827No Hit
CGTTATAAGGCAGCGGCAGCCCAACTGCCGGAGACCCTAGAGCCCATTAT11.7241379310344827No Hit
GAAATAGTCAGTGCAGCCAGATAAAAGTGACTCCGGACAGTGATCTTGGC11.7241379310344827No Hit
ATCATGGGATGAATATAAAAGAGTGAGCTTCACAATCTTGTGATCCATAT11.7241379310344827No Hit
CTGTTACTCACAAGGAGTGGGAAGCGGAGCTGGCCCCCTAGTGAAGTTCT11.7241379310344827No Hit
GTGTTACTCATAGCAGTAGCCATCTCCAGTGCAGTGCTCATGAGCACCGC11.7241379310344827No Hit
GTGTTGATATGTATCCTCTTGCAGCTATACTTGAGCGGTCTGTGAAGTGG11.7241379310344827No Hit
GTATAGCTGCAAGAGGATACATATCAACAAGGGTTGAAATGGGCGAGGCG11.7241379310344827No Hit
GAGCAAAAGCCGCGTGACAAAAACATAATGGACTCCGGCACAATGTCAAG11.7241379310344827No Hit
TCCTTGGCCCCATGGAACGTTATTTCTCTTTTGAGCTTCTTGTATAGTTT11.7241379310344827No Hit
ACATTGTAATATCAACTGGCATCTCCGTGATGGACGGAAACATCAACGCT11.7241379310344827No Hit
CCCAAAGATAACAAGATGGCAATTAAGATCAAGGAGGAAGTACGCCTTCA11.7241379310344827No Hit
CCAGTGAAACTGTTCCCAGCAAACCTAAGAGCATAATGGTCTCTAGGGTC11.7241379310344827No Hit
GGGGGTTATCACTACCATAGCTTCCATTCTTGATCACCAGCACATTGAAA11.7241379310344827No Hit
TGTGGGACTTGATCCCAGGATCCTGGGATCATGACCTGAGCCAAAGACAG11.7241379310344827No Hit
TGCCAAGTGAGCTACATCTCCTCCAGTGTTCATTTCTGCGAAAGTAGCAC11.7241379310344827No Hit
CTGTGACACTCTCCTCTCAGGTGGTGTTCTCTCTCCCTATCCACTAGGGC11.7241379310344827No Hit
GTGATCTTGGCACCAACTAGGGTTGTCGCTGCTGAGATGGAGGAGGCCTT11.7241379310344827No Hit
ACAGAATGATAGCTACTATCAGAGTCAGCGGTGATAATAGTCAAAAGCAA11.7241379310344827No Hit
GCAGCTGGCATCATGAAGAATCCCGTTGTGGATGGAATAGTGGTAACTGA11.7241379310344827No Hit
AGCTTTGGGTATGAATTTCCTTTTTTAACTAGCCATATTAAATTTTTGTA11.7241379310344827No Hit
AGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGGAACGTAC11.7241379310344827No Hit
GGCCTTGACAGTGATCATACACAAGGTGCTCAAGATGGCAATCAGAGGCA11.7241379310344827No Hit
ATACACTACTCCTCAAGCTAAACTAACAACACCAACACATTAACATTACA11.7241379310344827No Hit
TACATGAACTCCAACAACACAATAAATATAAATAATTAAACACAAATGGT11.7241379310344827No Hit
CTACTCCTTAATGGCGATGGCCACACAAGCTGGAGTGCTGTTTGGCATGG11.7241379310344827No Hit
ACACCCCTGACTCTGATAGTACCTATCATTCTGCTTGTGGCGCACTACAT11.7241379310344827No Hit
ATGATGGGTTGCTATTCACAATTAACACCCCTGACTCTGATAGTAGCTAT11.7241379310344827No Hit
ACTTAATGAGGTCAATGTTGACAGCGGGGTCAACCGATCATATGGACCAC11.7241379310344827No Hit
ATCATGAAGAATCCCGTTGTGGATGGAATAGTGGTAACTGACATTGACAC11.7241379310344827No Hit
AATGGTGACAGCGGGGTCAACCGATCATATGGCCAACTTCTCTCTTGGAG11.7241379310344827No Hit
GAAGGAGAGAAGAAGGAGCAACCATGGGATTCTAAATGGACATTGATCTG11.7241379310344827No Hit
ATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGGCACCCACGCT11.7241379310344827No Hit
AACCACACACCATTCAAGCAAGAAAAATTCTAAAAAACAAAAACAACAAG11.7241379310344827No Hit
GACATGGAGATGGATAATGAGATGAGTAAAAATTTTCAACTATTATGCGA11.7241379310344827No Hit
GTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTCTGCATTGTCT11.7241379310344827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATAG50.0144.01
AAATAGT50.0144.02
TAGTCAG50.0144.05
TCAGTGC50.0144.08
AATAGTC50.0144.03
ATAGTCA50.0144.04
GTCAGTG50.0144.07
CAGTGCA50.0144.09
AGTCAGT50.0144.06
Produced by FastQC (version 0.11.2)