FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_liver_ifn--_red_needle_pcr3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_liver_ifn--_red_needle_pcr3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569813
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCTATACAGTGACGAGAAACGCTGGCCTGGTTAAGAGACGTGGAGGTG25430.44628676425423774No Hit
GTATAGATAAGGGAAGCTCCTGCCAGATAGCTTCCTCTGAGGATGTTGCA16680.29272761414709736No Hit
ACATGTACTTGATCCCAGGCCTACAAGCGGCAGCAGCGCGTGCTGCCCAG13090.22972448856028205No Hit
GTATAAAGGTGCTGTGCCCATACACCAGCACTATGATGGAAACCATGGAG12370.21708876420860881No Hit
GTACCAGATGGCGCGGCTACCTTTTGCTTTCCCGAACTCTCCTTGCTTCT10160.17830411029583387No Hit
ATCTATGCCGCATTGACAACTCTCATCACCCCAGCTGTCCAACATGCGGT10070.17672464475187474No Hit
AATGGGAAGAGTGACTGACTGTTCACAGACCTCAGTGATGTGAGCACTGT9980.17514517920791559No Hit
ATATAAAATAGCTATTATAAATGCACATAGTGTATTCTATAGCTGCCAGG9560.16777434000277283No Hit
TACCAAAACAAAGTGGTGAAGGTTCTCAGACCAGCTGAAGGAGGAAAAAC9350.16408892040020148No Hit
GTATAGATAAGGGAAGCTCCTGCCAGATAGCTTCCTCTGAAGATGTTGCA9210.16163197399848722No Hit
ACCATGGGATTCTCAATGGACATTGATCTGCGGCCAGCCTCCGCCTGGGC8780.15408563862179347No Hit
TCACTGAAGTGTGTAGAGAGGAGGCTCGCCGTGCCCTCAAGGATGGAGTG8720.15303266159248735No Hit
ATACAGGACATGGGCCTACCATGGGAGCTACGAAGCCCCCACGCAAGGAT8680.15233067690628327No Hit
TCTATACAGTGACGAGAAACGCTGGCCTGGTTAAGAGACGTGGAGGTGGG8520.149522738161467No Hit
CTGTTACACTGTGGCTAAAACTTGTATCTTCAACCTTTGAAAAAGCCCAC8510.149347241989916No Hit
GGTGTGGACCCTTCCTCACCCAAGTAGCGGACTTGGGTGGATAGATAGTC8510.149347241989916No Hit
CCTATGATCCTGCGCACCATGTTGACTGTGTCTTTGATGTTTTCAGCCCA8350.1465393032450997No Hit
GAGTAACACTTGTCCCATCTTCTTCTCCACCTGGGGGTCTATTGTCATTG8150.14302937981407934No Hit
TTCTATCACAGTGATGTATGGTTAAACACTTGGATCAAGTCATAACCAGT8060.14144991427012021No Hit
ATTCCACACCATGAGCATGTCCTCAGTGGTCATCCATTCTCCCTTTCCAT7960.13969495255461No Hit
TCCCTTATCTATACAGTGACGAGAAACGCTGGCCTGGTTAAGAGACGTGG7730.13565854060893662No Hit
GTCCTGTATGGGTGGTTTTCATCAAGAAACCATGTTTCTGCATGTTCATT7670.1346055635796305No Hit
GGTTAAGAGACGTGGAGGTGGGACGGGAGAGACTCTGGGAGAGAAGTGGA7460.13092014397705914No Hit
ATCTGGTACATGTGGTTGGGAGCCAGATTCTTGGAGTTTGAAGCTCTTGG7450.13074464780550812No Hit
ATCTAATGGGAAGGAGAGAAGAAGGAGCAACCATGGGATTCTCAATGGAC7420.13021815929085506No Hit
CCTTAATGGCGATGGCCACACAAGCTGGAGTGCTGTTTGGCATGGGCAAA7370.1293406784331No Hit
GTATTTAATCACGGCCAACGCCAGAGTTCTGTGCCCTTCCTCCATTTGGT7360.12916518226154897No Hit
GTGTAGAGAGGAGGCTCGCCGTGCCCTCAAGGATGGAGTGGCCACAGGAG7320.1284631975753449No Hit
TTCTAGAAGAAATGAATCGGGCACCAGGAGGAAAGATGTACGCAGATGAC7240.12705922820293675No Hit
CTCCAGTGCTGTGCTGCTGCGGACCGCCTGGGGATGGGGGGAGGCTGGAG7100.12460228180122251No Hit
ATCCACTAGGGCCCAAAACCTTGGATCATTCACAGCTTCCACAGCCGTCT6990.12267182391416132No Hit
ATTAAATACACATACCAAAACAAAGTGGTGAAGGTTCTCAGACCAGCTGA6970.12232083157105927No Hit
AGTACATGTAGTGCGCCACAAGCAGAATGATAGCTACTATCAGAGTCAGG6760.11863541196848791No Hit
CTCTAACACTTCCTCAGCTTCCATGTTCCGGATAAGCTGCACCACCAAGT6550.11494999236591655No Hit
TCCCAGGAGGAGCTGACTTGTGGTGGACACACTTTTTATGATGTTGCTCT6380.11196655744954924No Hit
TTCCTAGATAGGGAATGTCTGTCCATTTTGTTACAGGAGTCTTGTCCTCC6370.11179106127799823No Hit
GTGTACAACATGATGGGAAAAAGAGAAAAGAAGCAAGGAGAGTTCGGGAA6270.11003609956248804No Hit
GAATAGTGGTAACTGACATTGACACAATGACAATAGACCCCCAGGTGGAG6130.10757915316077381No Hit
CCTTAATAGCTACCCAACAAGTCATTAACATACAGAAACATGCATCATGA6090.10687716847456973No Hit
ATCATAGAGAGCACTCTGAGTGTTCGTGTCTCTTCCACTTCAGGACTAGA6030.10582419144526362No Hit
CCATAAGTCCTCCCTTTTTCCTAGATAGGGAATGTCTGTCCATTTTGTTA5950.10442022207285548No Hit
GATAAGGGAAGCTCCTGCCAGATAGCTTCCTCTGAGGATGTTGCACAGTG5930.10406922972975344No Hit
CTACATGTACTTGATCCCAGGCCTACAAGCGGCAGCAGCGCGTGCTGCCC5920.10389373355820244No Hit
GACTAATCCTCCCCCATGCCTACGTTGCAGTCGCTCCATGGTTTCCATCA5900.10354274121510039No Hit
GTAATGAACATAGTCTCTTCCTGGCTGTGGAAGGAGCTGGGGAAACGCAA5870.10301625270044733No Hit
CTATAAGGGATCCACACCATAAGTCCTCCCTTTTTCCTAGATAGGGAATG5840.1024897641857943No Hit
GTTCATTACCTGGCGAGTGCCTTCTTGGGGATCTGGCACCCTGGTGTCCA5840.1024897641857943No Hit
GAGTAGAACTCCAGGGCCGACATCTGATTCAGACGAGCTTTCCACTTCTC5790.10161228332803919No Hit
ATCCCATCCATTGCTCTGCAACCATCTGGTCACTTTCTCTGGCTTCCTCA5770.10126129098493716No Hit
TTTCAAGACAAGACCAGAGAGGGAGTGGACAAGTTGTCACTTATGCTCTC5750.10091029864183514No Hit
GTGACTGACTGTTCACAGACCTCAGTGATGTGAGCACTGTGGCTCAGGAG5750.10091029864183514No Hit
CCTTTATACAGAAGGCCCCTGGTCTTTTTTCAAGCCAGTCCCCCATCATA5730.1005593062987331No Hit
TTCCTCACCCAAGTAGCGGACTTGGGTGGATAGATAGTCCATGTACTTTT5720.10038381012718207No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTAA2500.086.4076841
TCCCCAC5350.075.3711851
TCCGGGT608.4246494E-871.9937742
TGGACCC1950.070.147775
GTGGACC2800.064.280154
ATCTAAT5000.063.365641
CCCCACA6450.062.5062182
TCTAATG5350.059.2098272
TGTGGAC8050.059.025953
GACCCTT2150.056.925317
TAATGGG5950.053.239094
CTAATGG5950.053.239093
CCCACAC7800.051.6878363
CTCTATC1457.2759576E-1249.650883
CCCTTAA450.00990063248.0042721
TTGACGA450.009907547.9958466
ATTGACG450.009907547.9958465
AAGGACG450.009907547.9958468
CCGGGTG952.0142907E-645.469753
ACACCTT952.0142907E-645.469755
Produced by FastQC (version 0.11.2)