FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_liver_ifn--_red_needle_pcr2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_liver_ifn--_red_needle_pcr2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636406
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGAGATGGCTACTGCTATGAGTAACACTTGTCCCATCTTCTTCTC105141.6520900180073725No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT43030.6761407026332247No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA26120.4104298199576999No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG22840.35889039386806537No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC22700.356690540315459No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT20230.3178788383516183No Hit
GTTCAGACCATGCGGCCCTGAAGTCGTTCAAAGAATTCGCCGCTGGAAAA20200.3174074411617741No Hit
CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG20080.3155218524023972No Hit
TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTA19230.3021655986901443No Hit
GAGTAACACTTGTCCCATCTTCTTCTCCACCTGGGGGTCTATTGTCATTG18280.28723802101174406No Hit
ATCTATGCCGCATTGACAACTCTCATCACCCCAGCTGTCCAACATGCGGT17250.27105338416042585No Hit
GCTATGAGTAACACTTGTCCCATCTTCTTCTCCACCTGGGGGTCTATTGT16730.2628824995364594No Hit
GTGCCTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAGTACCG16220.2548687473091077No Hit
ACATGTACTTGATCCCAGGCCTACAAGCGGCAGCAGCGCGTGCTGCCCAG15600.24512653871899384No Hit
GTGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCT15530.24402661194269068No Hit
TCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAA15390.2418267583900843No Hit
AGATAAGGCCCAGCAGAGGTGTGGACAAAGTATGGAGAGAAGAGAGTGCT14800.2325559469898147No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA14730.2314560202135115No Hit
CAGCAGAGGTGTGGACAAAGTATGGAGAGAAGAGAGTGCTCAAACCGAGA13820.2171569721215702No Hit
AATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACCTTTTGCATAAT13590.2135429269994312No Hit
AGTACATGTAGTGCGCCACAAGCAGAATGATAGCTACTATCAGAGTCAGG13290.20882895510098898No Hit
TTATAAGGCAGCGGCAGCCCAACTGCCGGAGACCCTAGAGACCATTATGC13240.2080432931179153No Hit
CCTTAATGGCGATGGCCACACAAGCTGGAGTGCTGTTTGGCATGGGCAAA13100.20584343956530893No Hit
ATCTGTGCCTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAGT12710.1997152760973341No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT12630.19845821692441615No Hit
CTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAA11320.1778738729678853No Hit
GGTTAAAAGCTGTTTTGTTAATTATTCACTATTAAAGGTTTTTTCCGTTC11320.1778738729678853No Hit
AACTAGAAAACTTCTAACTAAAAGAATTACAGCTAGAAACCCCGAAACCA11060.17378843065590205No Hit
GGTATGAGCACCACCAGTAATAAAAACACAACAATGAGGACACATGCAAT10690.1679745319811567No Hit
GCATAAAATGGCATCCCTTTGCCCATGCCAAACAGCACTCCAGCTTGTGT10620.1668746052048535No Hit
ATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATAAT10570.1660889432217798No Hit
AACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGAACAAG10490.1648318840488619No Hit
GGTCTGAACAGACCCTAGCATCCATCCATCTCGGTTTGAGCACTCTCTTC10390.16326056008271447No Hit
GCTCATACCCGAGCCAGAGAAGCAAAGATCTCCCCAAGATAACCAGATGG10350.16263203049625552No Hit
GGAGATGGCTACTGCTATGAGTAACACTTGTCCCATCTTCTTCTCCACCT10290.1616892361165671No Hit
GTACATGTAGTGCGCCACAAGCAGAATGATAGCTACTATCAGAGTCAGGG9940.1561896022350512No Hit
ACCATGGGATTCTCAATGGACATTGATCTGCGGCCAGCCTCCGCCTGGGC9850.15477541066551856No Hit
TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA9230.14503320207540468No Hit
ATTTTATGTTGAGCTTGAACGCTTTCTTTATTGGTGGCTGCTTTTAGGCC8980.14110489216003622No Hit
ATCTGTGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGC8830.13874790621081512No Hit
ACCTAAAAACAATTTTATGAATCAACTCGTCTATGTGGCAAAATAGTGAG8700.13670518505482349No Hit
GGCTACTGCTATGAGTAACACTTGTCCCATCTTCTTCTCCACCTGGGGGT8520.13387680191575818No Hit
GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG8490.13340540472591397No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA8420.13230547794961078No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG8410.13214834555299604No Hit
GAATAAGGGCATCGGGAAGATGGGCTTTGGAATGGTAACCCTTGGGGCCA8380.13167694836315183No Hit
GGTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTT8380.13167694836315183No Hit
GATGAAAGACTAATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACC8330.13089128638007813No Hit
TCATACAACAACTACTCCTTAATGGCGATGGCCACACAAGCTGGAGTGCT8290.13026275679361918No Hit
ATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAATTGAAATTTTA8270.1299484920003897No Hit
ATTTTATGCATGGGACCTTGGAGTCCCGCTGCTAATGATGGGTTGCTATT8240.12947709481054545No Hit
TCCCATGGTTGCTCCTTCTTCTCTCCTTCCCATTAGATGAGCTATGTCAT8240.12947709481054545No Hit
ATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGAA8150.1280629032410128No Hit
GGTGTGGACAAAGTATGGAGAGAAGAGAGTGCTCAAACCGAGATGGATGG8110.12743437365455385No Hit
GCACTGGAGATGGGACATTGATGGTGCTGGTGAATTACATCTACTTTAAA8040.12633444687825068No Hit
CTATCAGAGTCAGGGGTGTTAATTGTGAATAGCAACCCATCATTAGCAGC8010.12586304968840648No Hit
CCATTAAGGAGTAGTTGTTGTATGAAGTGGTTACCGCATGTTGGACAGCT7870.1236631961358001No Hit
ATCTTGGGGAGATCTTTGCTTCTCTGGCTCGGGTATGAGCACCACCAGTA7860.12350606373918535No Hit
CCACTATTCCATCCACAACGGGATTCTTCATGATGCCAGCTGCTGTCCTT7770.12209187216965271No Hit
ACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAG7650.12020628341027582No Hit
GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT7380.11596370870167785No Hit
GAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGCTAGAAACCCCGA7290.11454951713214521No Hit
GATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAAT7240.11376385514907152No Hit
CATCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAAT7220.11344959035584203No Hit
CCCTTTGCCCATGCCAAACAGCACTCCAGCTTGTGTGGCCATCGCCATTA7170.11266392837276833No Hit
GTATGGAGAGAAGAGAGTGCTCAAACCGAGATGGATGGATGCTAGGGTCT7130.11203539878630937No Hit
GAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGAACAA7060.1109354720100062No Hit
CATCTAATGGGAAGGAGAGAAGAAGGAGCAACCATGGGATTCTCAATGGA7010.1101498100269325No Hit
TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC6980.10967841283708826No Hit
GCCCAGCAGAGGTGTGGACAAAGTATGGAGAGAAGAGAGTGCTCAAACCG6950.10920701564724405No Hit
CCCTTATTCCGCATCAAGACGAAGAAGATCCCCAGTGAAACTGTTCCCAG6890.10826422126755562No Hit
TTCTAGGTTTGATAACTGCAAACGAACTTGGATGGCTGGAAAGAACAAAA6830.10732142688786718No Hit
GCAGAGGTGTGGACAAAGTATGGAGAGAAGAGAGTGCTCAAACCGAGATG6680.10496444093864608No Hit
CTACATGTACTTGATCCCAGGCCTACAAGCGGCAGCAGCGCGTGCTGCCC6670.10480730854203135No Hit
CGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCC6650.10449304374880186No Hit
TTTCTGGGCAGCACGCGCTGCTGCCGCTTGTAGGCCTGGGATCAAGTACA6650.10449304374880186No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA6610.10386451416234291No Hit
AAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTTTA6480.10182179300635129No Hit
GTATGAAGTGGTTACCGCATGTTGGACAGCTGGGGTGATGAGAGTTGTCA6390.10040760143681864No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGAG27050.0110.4569243
TGGAGAT29000.0103.2779165
TCTGTGC8800.098.995352
CACTGGA34250.087.6571962
AGATAAG4650.085.170681
GGAGATG37650.082.035996
CTGGAGA37250.081.370684
CTGTGCC11750.079.655843
GATGCAC1100.078.541779
GCACTGG42300.071.156781
GATGGGG2200.062.17899
CGTCAGA350.00366569761.711392
ACGGTGT502.0800967E-457.5972988
TGTGCCT16500.056.724614
GAGATGG53150.054.0482647
GATGGTA1750.053.4832049
TGGACGG553.3307268E-452.361185
GAGAATT4750.051.5344248
GGAGAAT4850.050.4718557
GAGATAA1750.049.376871
Produced by FastQC (version 0.11.2)