FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_liver_ifn--_red_needle_f11_pcr1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_liver_ifn--_red_needle_f11_pcr1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129683
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTC401513.5541828990964723No Hit
GACAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAACAT293552.598516575003784No Hit
CTGACAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAAC224521.987460199011581No Hit
GCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATG180451.597350761231248No Hit
CATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAACATCGTGC171231.5157349451129212No Hit
ACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAACATCGTG167781.4851954043745015No Hit
TGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTG147311.3039941293265456No Hit
GATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGT136341.2068872418191652No Hit
GTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAG131861.1672300990631885No Hit
CGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTG114881.0169224463854019No Hit
GCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCA95390.844396171315316No Hit
GTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGC94750.8387308652073192No Hit
TGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCA86930.7695079062002349No Hit
GTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGG81900.7249821410076986No Hit
ATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAACATCGTGCC55970.49544872322589606No Hit
CACTGACAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACA53720.47553163143997035No Hit
GGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGAT53220.47110561104309795No Hit
GCCACGCACTGACAACATCGTGCCACGCACTGACAACATCGTGCCACGCA52490.4646436212636642No Hit
AATATACACATTGCACAGATGCACAGTGGAATATATGGATCGAAATCGGC52160.46172244780172844No Hit
GTGCCACGCACTGACAACATCGTGCCACGCACTGACAACATCGTGCCACG49630.4393267845935541No Hit
GCACTGACAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGAC49590.43897270296180435No Hit
TTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGT46550.41206249894882013No Hit
GTGCGTGGGAGACCAGACGGTTTCAATGATTGTTTAATGCAAACGACTCT45280.4008204071407642No Hit
CGCACTGACAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGA44470.39365025409783094No Hit
CAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAACATCG43040.3809918357627759No Hit
GGAATATATGGATCGAAATCGGCACCTAACTACATGCGGCTAATTGCGGT40580.35921581541016373No Hit
ATATACACATTGCACAGATGCACAGTGGAATATATGGATCGAAATCGGCA38750.34301658075761077No Hit
TGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGA36380.3220372440764356No Hit
GGTAATAGTAGTAGGCTTGAATTTCGCAAAAATCAGTAGTTCAAAAAAGT36210.320532397141499No Hit
TTTATTAACAGTAATTTTGGGTTGCCCTTAGCAACGGGTGTTTTGCAATT35860.31743418286368835No Hit
GTGGAATATATGGATCGAAATCGGCACCTAACTACATGCGGCTAATTGCG35700.31601785633668916No Hit
ACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTT30860.27317397889496436No Hit
ATATTACCCAGATCCAGTGCTGGGAATGGTAATAGTAGTAGGCTTGAATT29930.26494158095678166No Hit
TGACAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAACA29810.2638793360615323No Hit
ATATTAACTATCACCACTGGCAGAGATCTGGGCAGGGTTGGGAAAAGATC29490.261046683007534No Hit
GTTCTATCATATTACCCAGATCCAGTGCTGGGAATGGTAATAGTAGTAGG28580.2529913258852262No Hit
ACTGACAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAA27990.24776862181691678No Hit
GCCCTTAGCAACGGGTGTTTTGCAATTTTCAAGGCTCTTTTCCTACAGCA27450.24298851978829458No Hit
ATGTTGGGTGCATGAATTTTCTAAATCGTTAGTGTCATTGAAGGCTTTAA23050.2040395402958175No Hit
GTGCGTGGCAAACTAGATGTTGGTAACACGAACAGTAGCGACATTAGCAT21370.18916811176232623No Hit
AGCCTATGTTGGGTGCATGAATTTTCTAAATCGTTAGTGTCATTGAAGGC20400.18058163219239382No Hit
AACTAGATGTTGGTAACACGAACAGTAGCGACATTAGCATTCTAAGGTTA20290.17960790770508187No Hit
TCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAACATCGTGCCA20170.1785456628098325No Hit
TCCTACAGCAGCGATTGGCGTGAGTTTCACTGGCTTCGCTGACTGCGATT20060.17757193832252058No Hit
TGGTAATAGTAGTAGGCTTGAATTTCGCAAAAATCAGTAGTTCAAAAAAG19050.1686313771208383No Hit
GTCAGTGCGTGGGAGACCAGACGGTTTCAATGATTGTTTAATGCAAACGA18760.16606428529065234No Hit
GATCTGGGCAGGGTTGGGAAAAGATCTGAAATTCATTCTACAGTAGCTAA17810.15765484653659478No Hit
TAGTAGTAGGCTTGAATTTCGCAAAAATCAGTAGTTCAAAAAAGTGAATC17680.15650408123340795No Hit
TTGCACAGATGCACAGTGGAATATATGGATCGAAATCGGCACCTAACTAC17370.15375994858734707No Hit
TTCTAAGGTTAATGCTTCTACTGTTCTATCATATTACCCAGATCCAGTGC17040.1508387751254113No Hit
CTCCAAGAGCCATTGGTAAGTTCGTTGTTTTTAAGCAAATTAATGAACCA16480.1458816322809142No Hit
GTCTACTTAGGTAGGACCCCATAGGGGAACACGGCCCAATATACACATTG16190.14331454045072822No Hit
AACTACATGCGGCTAATTGCGGTTCTGAAAAACCTTAATTTTTCATAAAA16080.14234081596341627No Hit
ACTTAGGTAGGACCCCATAGGGGAACACGGCCCAATATACACATTGCACA15690.1388885200538558No Hit
GCCTATGTTGGGTGCATGAATTTTCTAAATCGTTAGTGTCATTGAAGGCT15690.1388885200538558No Hit
CTTTTCCTACAGCAGCGATTGGCGTGAGTTTCACTGGCTTCGCTGACTGC15510.13729515271098175No Hit
CCTAACTACATGCGGCTAATTGCGGTTCTGAAAAACCTTAATTTTTCATA15180.13437397924904598No Hit
CTATCATATTACCCAGATCCAGTGCTGGGAATGGTAATAGTAGTAGGCTT14230.12596454049498843No Hit
GTCAGTGCGTGGCAAACTAGATGTTGGTAACACGAACAGTAGCGACATTA14040.12428265274417691No Hit
GTTTAATGCAAACGACTCTGCTATGTCTACTTAGGTAGGACCCCATAGGG14020.124105611928302No Hit
GATGCACAGTGGAATATATGGATCGAAATCGGCACCTAACTACATGCGGC13920.12322040784892754No Hit
TGCTTCTACTGTTCTATCATATTACCCAGATCCAGTGCTGGGAATGGTAA13880.12286632621717775No Hit
GTAGTAGGCTTGAATTTCGCAAAAATCAGTAGTTCAAAAAAGTGAATCTC13860.12268928540130285No Hit
TTCTACAGTAGCTAAACAAAGTCAATCGCAGTCAGCGAAGCCAGTGAAAC13680.12109591805842877No Hit
CCATAGGGGAACACGGCCCAATATACACATTGCACAGATGCACAGTGGAA13490.11941403030761727No Hit
ACCCAGATCCAGTGCTGGGAATGGTAATAGTAGTAGGCTTGAATTTCGCA13440.11897142826793003No Hit
AAATAGAACATTTATTAACAGTAATTTTGGGTTGCCCTTAGCAACGGGTG12460.11029642829006013No Hit
GCCCAGATCTCTGCCAGTGGTGATAGTTAATATTTTGGTTCATTAATTTG12360.10941122421068564No Hit
GCTAAGGGCAACCCAAAATTACTGTTAATAAATGTTCTATTTCCCGCTGC12180.10781785686781159No Hit
GTAATAGTAGTAGGCTTGAATTTCGCAAAAATCAGTAGTTCAAAAAAGTG11970.10595892830112519No Hit
TCCCAACCCTGCCCAGATCTCTGCCAGTGGTGATAGTTAATATTTTGGTT11840.10480816299793835No Hit
AGTTAATATTTTGGTTCATTAATTTGCTTAAAAACAACGAACTTACCAAT11330.1002936221931285No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGTTG3250.079.7596361
ACCACGC1700.076.240821
AGGTCAT350.003665984161.7132953
ACACGAT5300.059.7779161
GGTCATA400.00621942353.9991384
TTCGACG1004.9663868E-850.4014242
GGGAGAC14700.046.0400777
TGGGAGA14900.045.4220926
GTCTGAA1151.491444E-743.8273242
CGACAGT1653.092282E-1143.639531
ATTCGAG855.5621596E-542.3560141
ACAGGTC700.00109290441.145841
GTTCGAC1452.048364E-839.727021
AGTCTGA2005.456968E-1239.602871
TCTGAAG1406.979499E-735.9994243
TCGAGTC1001.4495105E-435.9994243
CTGAGTA850.002836982533.8818134
CTGAAGC1501.1972443E-633.5994644
GGAGACC22250.033.3298078
TTCGAAC1751.0723852E-732.9152152
Produced by FastQC (version 0.11.2)