FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_infko_f-black_liver_pcr1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_infko_f-black_liver_pcr1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878676
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAAGGACTTCCTGTAGCTACTCCCAGGCTTACCATTCTGCAGTTGTAC52030.5921409029039145No Hit
CCCCCAGACTGAGTGGGGGCTCTGTAAGAGCAGGGCTGAGGAGCAGGGAC31000.35280353622950894No Hit
GAAGAGAGTATGGGCATCAGAAAAGAGTTGATCAGTCTTTCTTTACAAGC30980.35257592104484475No Hit
GTGCGTGGCTAAGGACTTCCTGTAGCTACTCCCAGGCTTACCATTCTGCA26380.30022442857207887No Hit
GTGCGTGGCTAAGAAAGGACACAGAAGAGAGTATGGGCATCAGAAAAGAG25540.29066459081618257No Hit
GTCAGTGCGTGGCTAAGAAAGGACACAGAAGAGAGTATGGGCATCAGAAA25390.288957476931201No Hit
GTCAGTGCGTGGCTAAGGACTTCCTGTAGCTACTCCCAGGCTTACCATTC18270.2079264711907461No Hit
GTGCGTGGCCAGCCACTTAGAATTATGTGGCTCTCTACTACCTTGGATCA17410.1981390182501855No Hit
ATCTAAGCTTGTCCATTTTTAGGCGGCATTTCAAGTGGCCAGAGAACAGC17070.1942695601108941No Hit
TCCTGTAGCTACTCCCAGGCTTACCATTCTGCAGTTGTACTCATTCCATG16880.19210721585658425No Hit
GTGCGTGGGCGGGGCCCAAAGTTCCATCCCGGCCAGGGAGTCTCCCAAGG16830.19153817789492372No Hit
GGTAATACAGATCTGAAAAGTCAAGGCCTGTCCTTGGTTCACAGTCAAGT16780.19096913993326323No Hit
GTATTACCTGACCATGAACAATAAGCATTGGTTGGTGCACAAAGAGTGGT16430.18698587420163973No Hit
ATGCTGGATGAGGGAGTGGAACCAGATGATGTCGATTGCTGGTGCAACAC15180.17275992516012728No Hit
AGTCTGAAGCGAGAGCTAACAACAGTATCAACAGGTTTAATTTGGATTTG15060.1713942340521421No Hit
CCTGTAGCTACTCCCAGGCTTACCATTCTGCAGTTGTACTCATTCCATGG14680.1670695455435223No Hit
GTCTTTCTTTACAAGCTAGATGGAGAGAAACATGAAGACAGCAAAGTTGC14560.16570385443553712No Hit
GCTAAGAAAGGACACAGAAGAGAGTATGGGCATCAGAAAAGAGTTGATCA13770.15671305464130125No Hit
GAGTATGGGCATCAGAAAAGAGTTGATCAGTCTTTCTTTACAAGCTAGAT13630.15511974834865183No Hit
GTATATGACTTTTTGGCTCGTCGAGCTTCCCAAAAGCCAGGCAATGGCAA13380.15227455854034935No Hit
GTCATAGGAGTTGGGGACAAGAAAATCACCCACCACTGGCATAGGAGTGG13260.15090886743236415No Hit
CTACATGTACTTGGATAGGAGCGATGCCGGGAAGGCCATTTCGTTTGCTA13020.14817748521639376No Hit
GAATATGACACGCCCTTCAATCTAAGCTTGTCCATTTTTAGGCGGCATTT12780.14544610300042338No Hit
TTCATATCCTGTATCATTGACAATCATCCCGCTATGCTGGGAGCCATGCA12740.14499087263109497No Hit
ACACAGAAGAGAGTATGGGCATCAGAAAAGAGTTGATCAGTCTTTCTTTA12710.14464944985409867No Hit
GTGCGTGGCCGGGTAGCTCCATCACGCGTGCGCGCACCTCGCGGCCTGAC12440.14157664486113197No Hit
GTCAGTGCGTGGGCGGGGCCCAAAGTTCCATCCCGGCCAGGGAGTCTCCC12210.13895907023749368No Hit
CTCATACAGTCTCCTGTTTCTCGAGCTCTCTGCAATTTCCTCATGCAACT11950.1360000728368591No Hit
GTAGTATGCACTCCCGCGTCTAGTGATCTCTGCTGCCATGGCTGTAGTCA11860.13497580450587018No Hit
CTCCCAGGCTTACCATTCTGCAGTTGTACTCATTCCATGGCTCCAGTTCC11860.13497580450587018No Hit
GTCAGTGCGTGGCCAGCCACTTAGAATTATGTGGCTCTCTACTACCTTGG11800.1342929589518776No Hit
GTCTTTAGCTGGTCCACTCAGCCACCACAACACCAGAGAGGGTTACAGAA11700.1331548830285566No Hit
CTACTACCTTGGATCATGAACCCCGAGACCTTCCTCTGTGGGGCCCGTTT11700.1331548830285566No Hit
GTCAGTGCGTGGCCGGGTAGCTCCATCACGCGTGCGCGCACCTCGCGGCC11420.12996827044325782No Hit
CTCTACTACCTTGGATCATGAACCCCGAGACCTTCCTCTGTGGGGCCCGT11250.12803354137361211No Hit
TCCTTGTAGAGTGAGAAGGGAGCGTCACGGCTCTTCTAGATCGCCGTGCC11060.12587119711930222No Hit
TTCCATGGCTCCAGTTCCAATCTAGGTGCAATTAAACTTTCCTCTCATAC11050.1257573895269701No Hit
TCTTAGAACACGTAAACTTGGCACATGTCACCAAGCTCCCTTTGCCAAAA11000.12518835156530964No Hit
GTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAACAGGTTTAATTTGG10860.12359504527266023No Hit
TAATAAAGAAGTTCAAGAAAGATCTTGCTGCCATGTTGAGAATAATCAAT10840.12336743008799604No Hit
GTGCGTGGCCACCCCCAGACTGAGTGGGGGCTCTGTAAGAGCAGGGCTGA10790.12279839212633553No Hit
CTCAATATGTCTGCAAAAGAACATTAGTGGACAGAGGTTGGGGAAACGGT10740.12222935416467504No Hit
GAGTTATGAGTGCCCTATGCTGGATGAGGGAGTGGAACCAGATGATGTCG10680.12154650861068243No Hit
TCCTAGAGGAGAATGGAGTTCAACTGACAGTTGTTGTGGGATCTGTAAAA10440.11881512639471203No Hit
GAAAAGAGTTGATCAGTCTTTCTTTACAAGCTAGATGGAGAGAAACATGA10340.11767705047139106No Hit
GTATCAACAGGTTTAATTTGGATTTGGAAACGAGAGTTTCTGGTCATGAA10260.11676658973273424No Hit
TTAGTGGACAGAGGTTGGGGAAACGGTTGTGGACTTTTTGGCAAAGGGAG10220.11631135936340585No Hit
TCCGTACACAACCCAAGTTGATGTCGTGTTGCACCAGCAATCGACATCAT10210.11619755177107376No Hit
CTCCAAGAGAGAAGTGGTCCATATGATCGGTTGACCCCGCTGTCACCATT10100.11494566825542066No Hit
GTTAGTAACATGGCCGAGGTAAGATCCTATTGCTACGAGGCATCGATATC10100.11494566825542066No Hit
GTATGGGCATCAGAAAAGAGTTGATCAGTCTTTCTTTACAAGCTAGATGG10040.11426282270142805No Hit
GTCTAAGATATTTATACTCCCCACTGTGGCCAGCAAACATAACTGGTGTG9940.11312474677810705No Hit
GTCAGTGCGTGGCCCTCAGGCGAATAGATGCACAGAGCAGCCAGCAATTC9860.11221428603945026No Hit
CATATTGAGTGTCTGATTGCTTGTCAAGGTAGGCCTCACCTTGTGTTGGG9860.11221428603945026No Hit
GGATAGGAGCGATGCCGGGAAGGCCATTTCGTTTGCTACCACATTGGGAG9820.11175905567012187No Hit
TCCATGCTCTGTGCTCAAGCGGACATTCCTTCAGTGTGTCACCGTCGACA9750.11096240252379717No Hit
TCCCTTAACAGCTGTTCCTATGACGGCTGGGTCACATTCTAATGAGTAAT9700.11039336456213668No Hit
GTGCGTGGCACGGCCATTGCTCGAATTGCCAACCAGGCCAAAGCAAATCC9670.11005194178514037No Hit
ATCTAGAAGAGCCGTGACGCTCCCTTCTCACTCTACAAGGAAGTTGCAAA9600.10925528863881567No Hit
ATATCGGACATGGCTTCGGACAGTCGTTGCCCAACACAAGGTGAGGCCTA9590.10914148104648358No Hit
CTTCTAGATCGCCGTGCCTCACCTTTTTTGTGATGACAGGTTCCGTACAC9390.10686532919984158No Hit
GCTAAAGACTTGGGTATGATAAGATCACTTTCTTCTACTCCATCTGTCCA9330.10618248364584898No Hit
TTATAGATGAATACCCCCGTGCCACATCTCGTTTCCTTTTTTGAGAAGTC9230.10504440772252797No Hit
ACTCTATGTCAACTGTTGGCTTGTCCTGTGCCATCACGGTAACGCAGCCT9070.1032234862452144No Hit
GTACAAGTACCATCCTGACTCCCCCCGCAGATTGGCAGCAGCAGTCAAGC9070.1032234862452144No Hit
ACCTTGGATCATGAACCCCGAGACCTTCCTCTGTGGGGCCCGTTTTCCTG8890.1011749495832366No Hit
AGAAAGGACACAGAAGAGAGTATGGGCATCAGAAAAGAGTTGATCAGTCT8870.1009473343985724No Hit
CTACAAGGAAGTTGCAAACGCGGTCGCAGACCTGGTTAGAATCAAGAGAA8790.10003687365991559No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAGTG16350.0106.131381
CAGTGCG16450.0105.9178853
AGTGCGT16750.0104.020844
TCGAGTC1950.092.3049853
GTTCGAG2000.090.002491
TTCGAGT2050.087.802312
CGAGTCT2250.076.797754
ACTGAGT7750.072.4623958
CCCAGAC7850.071.5393143
CCAGACT8000.071.097924
GACTGAG7950.070.639447
CAGACTG8150.069.789375
TGCGTGG33050.066.224992
GTGGCTA11600.065.170515
CGTGGCT11950.064.4667364
GCTAAGG12250.061.7159921
TAAGGAC12600.060.569663
TGGCTAA12600.059.9982456
CTAAGGA12750.059.8570752
GGCCACC3150.059.4268347
Produced by FastQC (version 0.11.2)