HVHNVAFXX_n01_infko_f-black_brain_pcr2.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HVHNVAFXX_n01_infko_f-black_brain_pcr2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	357323
Sequences flagged as poor quality	0
Sequence length	150
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

$Per base sequence content$

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCACTGGAGATGGCTACTGCTATGAGTAACACTTGTCCCATCTTCTTCTC	4275	1.1963965375864414	No Hit
CAGTATGACACCAAGTCCTGCTTGACATCCCCCCAGTATGGATCAAGTCT	985	0.27566095661348416	No Hit
CTATTAATGTGGTAGGACTACTGTTACTCACAAGGAGTGGGAAGCGGAGC	939	0.26278745001021486	No Hit
GGTCTGAACAGACCCTAGCATCCATCCATCTCGGTTTGAGCACTCTCTTC	919	0.2571902732261847	No Hit
TACTACAACCCATCAGAGTCCCTAATTACAATCTCTACATCATGGATGAA	901	0.25215281412055757	No Hit
CATTAATAGGGTCTACTACCCTCACAGCTGTCAATCCCAGGGCCATGACA	869	0.24319733126610937	No Hit
GGATGAGAGTGGTGATTTCTCCTTGGTAGAGGAAGATGGTCCACCCATGA	860	0.2406786017132958	No Hit
CCTTTTGTAACGTGCCACATGGTGTGGAAGACTCCCTCTTGCATGACTCC	823	0.23032382466284007	No Hit
GTTCAGACCATGCGGCCCTGAAGTCGTTCAAAGAATTCGCCGCTGGAAAA	795	0.22248777716519785	No Hit
CTCTAGGAGATGCCTAAAACCAGTCATACTTGATGGTGAGAGAGTCATCT	788	0.22052876529078733	No Hit
TGCTAGTGCTGCTCAGAGGAGAGGACGTATAGGCAGGAACCCTAACAAAC	786	0.21996904761238434	No Hit
TATGTGCACTGGCCGGAGGGTTTGCCAAGGCAGACATTGAGATGGCTGGA	681	0.1905838694962261	No Hit
CCCCGTGAGAGCATGCTGCTAGCCCTGGCTTCGTGTCTTCTGCAAACTGC	668	0.18694570458660653	No Hit
GAGTAACACTTGTCCCATCTTCTTCTCCACCTGGGGGTCTATTGTCATTG	668	0.18694570458660653	No Hit
CTCCTAGAGTCTATGACCCGGTCAGCCTTGAAGTTGGCGCCCATCTCTGA	641	0.17938951592816585	No Hit
TACACATACCACGCTCCTGCAGCAAAAGGTATAGCTATTGGGTTCATGCC	629	0.17603120985774776	No Hit
AGATAAGGCCCAGGAAAGAACCAGAGAGCAACTTAGTGAGGTCAATGGTG	608	0.1701541742345161	No Hit
GATATGTGCACTGGCCGGAGGGTTTGCCAAGGCAGACATTGAGATGGCTG	607	0.16987431539531458	No Hit
CTCTCATCCAGTGCCACGTCAAGCCGAGGACTGTTTCCAGTGACTTCCGC	598	0.16735558584250104	No Hit
GTATGACACCAAGTCCTGCTTGACATCCCCCCAGTATGGATCAAGTCTTC	594	0.166236150485695	No Hit
CTCCCTCTTGCATGACTCCCACTCCAACCTGTGTTGAACCTAGCAGTCTG	592	0.165676432807292	No Hit
TATCAGGCCAACGGCTGTGAGAACTTCACTAGGGGGCCAGCTCCGCTTCC	587	0.16427713861128448	No Hit
CATGTATGGAGGTGGGTGTGCAGAGACTGATGAAGGCCATGCACACTGGC	576	0.1611986913800679	No Hit
CTTCCACACCATGTGGCACGTTACAAAAGGAGCCGCACTGAGGAGCGGTG	556	0.15560151459603777	No Hit
ATCTATGCCGCATTGACAACTCTCATCACCCCAGCTGTCCAACATGCGGT	550	0.15392236156082872	No Hit
TCTCTGAGATGTCAGTTGTTATGACAAAGTCCCACTCTTGATTTTTTGTT	543	0.15196334968641817	No Hit
GTACAGAGTGATGACTCGCAGACTGCTAGGTTCAACACAGGTTGGAGTGG	541	0.15140363200801515	No Hit
GCTATGAGTAACACTTGTCCCATCTTCTTCTCCACCTGGGGGTCTATTGT	522	0.14608631406318653	No Hit
TCTCAGAGATGGGCGCCAACTTCAAGGCTGACCGGGTCATAGACTCTAGG	522	0.14608631406318653	No Hit
GTATGGAGGTGGGTGTGCAGAGACTGATGAAGGCCATGCACACTGGCTTG	512	0.14328772567117146	No Hit
TACTCAAGGTGGTCCTGATGGCCATCTGTGGCATGAACCCAATAGCTATA	510	0.14272800799276844	No Hit
GTACACTCCATCTGTGGTCTCTCCTTTCTTCACTTCTTTGGGAGCAGGCA	508	0.14216829031436543	No Hit
CTCCCACTCCAACCTGTGTTGAACCTAGCAGTCTGCGAGTCATCACTCTG	494	0.13825026656554434	No Hit
CTCTTGGAGTGCTTGTGATTCTACTCATGGTGCAGGAGGGGTTGAAGAAG	492	0.13769054888714133	No Hit
ACCCTGCAGGGACCTCAGGATCTCCGATCCTAGACAAATGTGGAAGAGTG	487	0.1362912546911338	No Hit
CACATACCACGCTCCTGCAGCAAAAGGTATAGCTATTGGGTTCATGCCAC	476	0.13321280745991723	No Hit
GTGTATGTGAAGACTGGGAAAAGGAGTGGCGCCCTCTGGGACGTGCCTGC	471	0.13181351326390967	No Hit
GTGTGGAAGACTCCCTCTTGCATGACTCCCACTCCAACCTGTGTTGAACC	470	0.13153365442470816	No Hit
CTCTACATCATGGATGAAGCCCACTTCACAGACCCCTCAAGTATAGCTGC	455	0.12733577183668557	No Hit
GTATGTGTATGTGAAGACTGGGAAAAGGAGTGGCGCCCTCTGGGACGTGC	452	0.12649619531908105	No Hit
AGTACATGTAGTGCGCCACAAGCAGAATGATAGCTACTATCAGAGTCAGG	451	0.12621633647987957	No Hit
CATTAATGGATTTGCTTTGGCCTGGTTGGCAATTCGAGCAATGGCCGTGC	442	0.12369760692706598	No Hit
GGCCTGATATGTGCACTGGCCGGAGGGTTTGCCAAGGCAGACATTGAGAT	442	0.12369760692706598	No Hit
GCCATAGAGTCCTATCACTCTTCCACATTTGTCTAGGATCGGAGATCCTG	435	0.12173859505265544	No Hit
TTACAAAAGGAGCCGCACTGAGGAGCGGTGAGGGAAGACTTGATCCATAC	434	0.12145873621345395	No Hit
GTGTAAGTTATTCCGGCAGATGCAACCTGATAGGCTAGCCAGACGGGAAG	419	0.11726085362543134	No Hit
GGCTGGACCCATGGCTGCAGTAGGCTTGCTAATTGTCAGCTATGTGGTCT	411	0.11502198291181928	No Hit
GTCTTCCATTATGGTGTTGTTGGTTGTGCCATCAAAGCACCATCTTCTGT	401	0.1122233945198042	No Hit
GCACGCGCTGCTGCCGCTTGTAGGCCTGGGATCAAGTACATGTAGTGCGC	400	0.1119435356806027	No Hit
CTCTAACACCACTAGCTCGAGGCACACTGCTCGTGGCATGGAGAGCGGGC	399	0.11166367684140119	No Hit
GATGTAGAGATTGTAATTAGGGACTCTGATGGGTTGTAGTAAGCGTGAAG	397	0.11110395916299819	No Hit
AACTTACACAGACAGAAGATGGTGCTTTGATGGCACAACCAACAACACCA	392	0.10970466496699066	No Hit
GCCTTGAAGTTGGCGCCCATCTCTGAGATGTCAGTTGTTATGACAAAGTC	391	0.10942480612778915	No Hit
GTGCAGGAGGGGTTGAAGAAGAGAATGACCACAAAGATCATCATGAGCAC	390	0.10914494728858763	No Hit
TTATAAGGCAGCGGCAGCCCAACTGCCGGAGACCCTAGAGACCATTATGC	390	0.10914494728858763	No Hit
CTTGTGGAGGGATCATGCTCCTCTCCCTGAAAGGGAAAGGTAGTGTGAAG	385	0.10774565309258011	No Hit
GGAGTGTACAGAGTGATGACTCGCAGACTGCTAGGTTCAACACAGGTTGG	385	0.10774565309258011	No Hit
CCTTAATGGCGATGGCCACACAAGCTGGAGTGCTGTTTGGCATGGGCAAA	384	0.1074657942533786	No Hit
GCCCAGGAAAGAACCAGAGAGCAACTTAGTGAGGTCAATGGTGACAGCGG	383	0.10718593541417709	No Hit
GTATGGATCAAGTCTTCCCTCACCGCTCCTCAGTGCGGCTCCTTTTGTAA	379	0.10606650005737107	No Hit
ATCATACTCAAGGTGGTCCTGATGGCCATCTGTGGCATGAACCCAATAGC	379	0.10606650005737107	No Hit
CATCAATGGCAGTGCTGGTAGTCATGATCTTGGGAGGATTTTCAATGAGT	378	0.10578664121816957	No Hit
GCCTTATAAGGCCTGCTTCCAGTCTCTGCTCGCATGAGCACGGCGAGGTT	375	0.10494706470056503	No Hit
GTTGTAGTAAGCGTGAAGTGAAAGTGGCATGGCACATCAAATCAACGATT	371	0.10382762934375901	No Hit
CCATACATGTACTCATCTCCAGGTTTGTTAGGGTTCCTGCCTATACGTCC	370	0.1035477705045575	No Hit
GCTATTGGGTTCATGCCACAGATGGCCATCAGGACCACCTTGAGTATGAT	368	0.10298805282615449	No Hit
CCCACCTCCATACATGTACTCATCTCCAGGTTTGTTAGGGTTCCTGCCTA	362	0.10130889979094544	No Hit
TCCCAGAGGGCGCCACTCCTTTTCCCAGTCTTCACATACACATACCACGC	362	0.10130889979094544	No Hit
ATGTTAGTGCTATAACCCAGGGAAAGAGGGAGGAGGAGACTCCGGTTGAA	360	0.10074918211254243	No Hit
GGTATGAGCACCACCAGTAATAAAAACACAACAATGAGGACACATGCAAT	360	0.10074918211254243	No Hit
CATATCAACAAGGGTTGAAATGGGCGAGGCGGCTGCCATTTTTATGACTG	359	0.10046932327334092	No Hit
ATCATGGACACAGAAGTGGAAGTCCCAGAGAGAGCCTGGAGCTCAGGCTT	359	0.10046932327334092	No Hit
ATGGAAGACAGCGTACCAGCAGAGGTGTGGACAAAGTATGGAGAGAAGAG	358	0.10018946443413942	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTCAG	20	2.2033091E-6	144.0	1
GTCAGAT	30	1.6539141E-5	96.0	3
CGTCAGA	30	1.6539141E-5	96.0	2
GCCTGCA	40	6.366299E-7	90.0	3
TCAGATG	35	3.554169E-5	82.28571	4
ACTGGAG	1290	0.0	73.67442	3
AGATAAG	130	0.0	72.0	1
GATGGTA	30	0.001988569	72.0	9
GATGGGG	40	6.889527E-5	72.0	9
GAGATAA	70	2.9631337E-9	72.0	1
CTGGAGA	1465	0.0	65.36518	4
TGGAGAT	1480	0.0	65.189186	5
TCTGTGC	95	4.838512E-10	60.631577	2
AACAGGC	40	0.0062155654	54.0	1
CAGATGT	60	5.1122985E-4	48.0	5
AGATGTG	65	7.584385E-4	44.307693	6
TACTACA	475	0.0	40.92631	1
GGAGATG	2610	0.0	40.551723	6
ACTACAA	485	0.0	40.082474	2
TACAACC	500	0.0	38.879997	4

Summary