FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_infar_m-black_blood_pcr3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_infar_m-black_blood_pcr3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586698
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGATAAGGGAAGCTCCTGCCAGATAGCTTCCTCTGAAGATGTTGCA23850.40651237945246105No Hit
TATCTATACAGTGACGAGAAACGCTGGCCTGGTTAAGAGACGTGGAGGTG15500.26419043528357006No Hit
GTATAAAGGTGCTGTGCCCATACACCAGCACTATGATGGAAACCATGGAG11260.1919215678253548No Hit
ACATGTACTTGATCCCAGGCCTACAAGCGGCAGCAGCGCGTGCTGCCCAG9610.16379806987581344No Hit
CCTTTATACAGAAGGCCCCTGGTCTTTTTTCAAGCCAGTCCCCCACCATA7870.13414056294720622No Hit
GATAAGGGAAGCTCCTGCCAGATAGCTTCCTCTGAAGATGTTGCACAGTG7660.13056120866271917No Hit
GAGTAACACTTGTCCCATCTTCTTCTCCACCTGGGGGTCTATTGTCATTG6850.11675512785112613No Hit
ATACAGGACATGGGCCTACCATGGGAGCTACGAAGCCCCCACGCAAGGAT6820.11624379152477085No Hit
CTGTATAGATAAGGGAAGCTCCTGCCAGATAGCTTCCTCTGAAGATGTTG6770.11539156431417867No Hit
GTCCTGTATGGGTGGTTTTCATCAAGAAACCATGTTTCTGCATGTTCATT6130.10448305601859902No Hit
ATTCCACACCATGAGCATGTCCTCAGTGGTCATCCATTCTCCCTTTCCAT6090.10380127425012528No Hit
GGTGTGGACATCCCCAAGGTTAAGTGGTCTGCCCCGTATAAGGTCAGGAG5950.10141503806046723No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAAT3500.078.189341
GAAGGCG1250.069.118169
TGTGGAC15100.067.70683
TCCGGGT2150.063.6370622
CATCTAA2250.060.813931
ACGATGC400.006218201553.998568
CTAATGG5250.053.484293
TGGACGC953.3129254E-853.0512245
GTGGACT5450.052.842634
GACTCGA859.356172E-750.8221747
TCTAATG5550.050.601872
CGATGCG450.00990530147.9987269
GGACGCT605.1156734E-447.998726
TAATGGG6050.047.602044
GGACTCG952.013563E-645.4724776
ACTCGAC952.013563E-645.4724778
TGGACGA803.889074E-544.9988025
CTCCGGG3150.043.4385221
CCGGGTG3150.043.4274143
GTAGAAG1002.8659433E-643.198853
Produced by FastQC (version 0.11.2)