FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_female_transmission_blood_pcr3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_female_transmission_blood_pcr3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254095
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGATAAGGGAAGCTCCTGCCAGATAGCTTCCTCTGAAGATGTTGCA8320.32743658867746317No Hit
TATCTATACAGTGACGAGAAACGCTGGCCTGGTTAAGAGACGTGGAGGTG5620.22117711879415178No Hit
GTATAAAGGTGCTGTGCCCATACACCAGCACTATGATGGAAACCATGGAG3620.1424664003620693No Hit
ACATGTACTTGATCCCAGGCCTACAAGCGGCAGCAGCGCGTGCTGCCCAG3510.13813731084830477No Hit
GATAAGGGAAGCTCCTGCCAGATAGCTTCCTCTGAAGATGTTGCACAGTG2970.1168854168716425No Hit
GTATCAGGAAACTCTGCAAACCAGGAAGTAATCCACAGAACCAAGCAAAG2730.10744013065979259No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTAA950.083.366831
GTGGACT1600.067.498714
ACTATAC451.2337987E-463.998783
TGTGGAC6300.058.28463
GTACAAT502.077423E-457.5988966
TGATCCG400.00621397753.9989667
ATCCGTC400.00621397753.9989669
ATCTAAT1700.050.8225561
TCTAATG2150.050.2315982
GGACTAT752.6517459E-547.999086
GTGGACC2250.044.799144
CTAATGG2000.043.1991733
ATTATAC700.001091796141.142073
GAAGGCG700.001091796141.142079
GTATAGA4250.037.2698751
CTATACA3300.037.09024
TAATGGG2351.8189894E-1236.765254
TATAGAT4550.036.3949052
TGGACTA1406.951359E-735.9993135
ATCTATA3000.035.999312
Produced by FastQC (version 0.11.2)