FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_demux_negative_control_pcr3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_demux_negative_control_pcr3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC19.090909090909092No Hit
GAATACACGAAGCACTTGATCAAGGTTGAAAACTGGATATTCAGGAACCC19.090909090909092No Hit
CTATATGAACAGCTAGGCAAAAAATTCTCGAATAGATTCCAACAATTCTG19.090909090909092No Hit
ACTAAGAGGGGTAGCCCCATTGCATTTGCGTAAATGTAACATTGCTGGCT19.090909090909092No Hit
GTCAACTGGCACAGCCGAGCAAATGGCATTAGCCATCAGTCGAAGGTCTC19.090909090909092No Hit
ATCCAAATGTGCACTGAACTCAAACTCAGTGATTATGAGGGACGACTAAT19.090909090909092No Hit
CAAGTGTTGTTTTCATAAGTAATGACGCTTTGATTGCATCTTTCAAACTG19.090909090909092No Hit
GGATATGTAACACAACAGGGGGAGAGAAGCCTAATTTTGTTCCTGCTTAG19.090909090909092No Hit
GTGACAAGTTTACGTGTTCTAAGAAGATGACCGGGAAGAGCATTCAACCG19.090909090909092No Hit
GCCCCCTACTGGGAGCCCAGACTGTTCAAGCTTTTCGCAAATGCTCCTAG19.090909090909092No Hit
ATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGACATTTT19.090909090909092No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)