FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_demux_negative_control_pcr2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_demux_negative_control_pcr2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50
Sequences flagged as poor quality0
Sequence length150
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACATCATGGATGAAGCCCACTTCACAGACCCCTCAATTATAGCTGCAA12.0No Hit
GATTGAAGGGCGTGTCATATTCCTTGTGCACTGCGGCATTCACATTCACC12.0No Hit
GGATGGATGCTAGGGTCTGTTCAGACCATGCGGCCCTGAAGTCGTTCAAA12.0No Hit
GCTCGATGCCATCCCCGATGGGGTCTTGTCCGAGATCCGGGGGTGCGTGC12.0No Hit
GCTTGACATACCCCCAGTATGGATCAAGTCTTCCCTCACCGCTCCTCAGT12.0No Hit
TACAAGCCTTTTGGGAAAATCGAATAGCAAAAAAGTCAAATAAATCTATA12.0No Hit
GCACAAAAAAAGCAAGCCTCATTCAGCACTTCAAAAGGGCAACACAAGCT12.0No Hit
ACCGGAGTCTCCTCCTCCCTCTTTCCCTGGGTTATAGCACTAACATAGCT12.0No Hit
GTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAACAGGTTTAATTTGG12.0No Hit
ATTATGGAGAGAAGAGAGTGCTCAAACCGAGATGTATGGATGCTAGGGTC12.0No Hit
CTGCGATCTCTGCTCTTGAAGGTGACTTGATGGTCCTCATTAAAGGATTT12.0No Hit
GCTTATTGTTCATGGTCAGGTAATACAGATCTGAAAAGTCAAGGCCTGTC12.0No Hit
CCATGAGTAGAATCACAAGCACTCCAAGAGAGAAGTGGTCCATATGATCG12.0No Hit
CTCCATGGTAGAGGAAGATGGTCCACCCATGAGAGAGATCATACTCAAGG12.0No Hit
GGGTGGGAGAGAAATCGTTGATTTGATGTGCCATGCCACGTTCAATTCAC12.0No Hit
TTTCAGTAGCACCCCTCCCCCCATCTCTCTCTCTGGCTCACAAGTTCCCT12.0No Hit
ATATCGGACATGGCTTCGGACAGTCGTTGCCCAACACAAGGTGAGTAAAA12.0No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG12.0No Hit
GAAGAAGAGAATGACCACAAAGATCATCATGAGCACATCAATGGCAGTGC12.0No Hit
GCTATTCACAATTAACCCCCCTGCCTCTGCTAGTAGCTATCATTCTTCTA12.0No Hit
CCATACATGTACTCATCTCCAGGTTTGTTAGGGTTCCTGCCTATACGTCC12.0No Hit
CACCGCTCCTCAGTGCGGCTCCTTTTGTAACGTGCCACATCGTGTTGAAG12.0No Hit
GTAACATATCTATCATGGCTCGACCAACTTCATCTACAAACAACAAATAC12.0No Hit
CTTGTCCAAGGGGATGGCACATGCTCGTTAGCTTGACATGTCAAAACACT12.0No Hit
AAGATGATGTTGGAGCTAGACCCACCAATTGGGGATTCTTACATTGTCAT12.0No Hit
GGAAGACCTTCGTGGAACTCATGAAGAGAGGAGACCTTACCGTCTGGCTA12.0No Hit
AATCCTGGCTGCTCTAACACCACTAGCTCGAGGCACACTGCTCGTGGCAT12.0No Hit
GGTCACTCATTGAAAATCCTCCCAAGATCATGACTAAAATCAAATTCAAA12.0No Hit
AGTCACCTTCAAGAGCAGAGATCGCAGTTTGCAGAAGACACGAAGCCAGG12.0No Hit
TGTACATTGAAAGAGCAGGTGACATCACATGGGAAAAGGACGCGGAAGTC12.0No Hit
GGAAAGAGAGGAAGAGACGTGGCGCAGACACCAGCATCGGAATCATTGGC12.0No Hit
GCATACTACATGTACTTGGATAGGAGCGATGCCGGGAAGGCCATTTCGTT12.0No Hit
AGGTGGTCCTGATGGCCATCTGTGGCATGAACCCAATAGCTATACCTTTT12.0No Hit
CAGAACAGCTTTCCCTTTGCACCATCCATCTAAGCCTCTAGAGCTCCAGC12.0No Hit
ATCTCCATTCCATACCACCAGCCCTCTTTTGCTCGAAACGATAGAGCCCC12.0No Hit
GGGAAAAGCAGGGGACCTGCCAAACAGGGAGGTCCCTGATGTGGGGCTGG12.0No Hit
GGCATAGATAGCCCAGGCGGAGGCTGGCCGCAGATCAATGTCCATTGAGA12.0No Hit
TCTTCAACCCCTCCTGCACCATGAGTAGAATCACAAGCACTCCAAGAGCT12.0No Hit
GGGAAAAGGAGTGGCGCCCTCTGGGACGTGCCTGCTCCCAAAGAAGTGAA12.0No Hit
GGTGTGCAGAGACTGATGAAGGCCATGCACACTGGCTTGAAGCAAGAATG12.0No Hit
ATAACTATACCTTTTGCTGCAGGAGCGTGGTATGTGTATGTGAAGACTGG12.0No Hit
CCCCCGACTGATCCGAAGTCCCAGGCTGTATCCCCCAGGACTGCCATTCT12.0No Hit
TCTTCACACTACCTTTCCCTTTCAGGGAGAGGAGCATTATAACTCCACAA12.0No Hit
GTCAAGGCCTGTCCTTGGTTCACAGTCAAGTCCTAAGCTTCCAAAGACTC12.0No Hit
GAAGACACGAAGCCAGGGCTAGCAGCATGCTCACACGGGGTGTCCAATTG12.0No Hit
GCATAGGAGTGGTAGCACCATCGGAAAGGCATTTGAGGCCACTGTGAGAG12.0No Hit
GGTTTTTCGGAAGCTCGACGAGCCAAAAAGTCATATACTTGGTCATGATA12.0No Hit
GTCACATTCAAGAGCAGAGATCGCAGTTTGCAGAAGAAACGAAGACATGG12.0No Hit
GATGAAGGCCATGCACACTGGATTGAAGCAAGAATGATTCTTGACAACAT12.0No Hit
AATGAGGACCATCAAGTCACCTTCAAGAGCAGAGATCGCAGTTTGCAAAA12.0No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTGG50.0144.02
AATCCTG50.0144.01
GGCTGCT50.0144.07
CTGGCTG50.0144.05
GCTGCTC50.0144.08
CTGCTCT50.0144.09
TCCTGGC50.0144.03
TGGCTGC50.0144.06
CCTGGCT100.072.04
Produced by FastQC (version 0.11.2)