FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_demux_negative_control_pcr1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_demux_negative_control_pcr1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGTGGACAGATGGAGTAGAAGAAAGTGATCTTATCATACCCAAGTCTT22.73972602739726No Hit
GTTTGGAGGAATGTCCTGGTTCTCACAGATCCTCATAGGCACGCTGCTAG11.36986301369863No Hit
CCGCTATGCTGGGAGCCATGCACTGATAGCATTATCCGATACTCCAGATT11.36986301369863No Hit
CATCGTTATAGATGAATACCCCCGTGCCACATCTCGTTTCCTTTTTTGAG11.36986301369863No Hit
TGTCACCAAGCTCCCTTTGCCAAAAAGTCCACAACCGTTTCCCCACCCTC11.36986301369863No Hit
AGTGGACAGAGGTTGGGGAAACGGTTGTGGACTTTTTGGCAAAGGGAGCT11.36986301369863No Hit
GGAATGTCCGCTTGAGCACAGAGCATGGAATAGTTTTCTTGTGGAGGATC11.36986301369863No Hit
CCATTGATGTGCTCATGATGATCTTTGTGGTCATTCTCTTCTTCAACCCC11.36986301369863No Hit
CATTCACCAAGGTCCCAGCTGAAACACTGCATGGAACAGTCACAGTGGAG11.36986301369863No Hit
GTGAATGAGCTGCCCCATGGCTGGAGAGCCTGGGGGAAATCGTATTTTGT11.36986301369863No Hit
GAAGGAATGTCCGCTTGAGCACAGAGCATGGAATAGTTTTCTTGTGGAGG11.36986301369863No Hit
GACATATTGAGTGTCTGATTGCTTGTCAAGGTAGGCCTCACCTTGTGTTG11.36986301369863No Hit
CCAATGCTTATTGTTCATGGTCAGGTAATACAGATCTGAAAAGTCGAGGC11.36986301369863No Hit
TAAAGAGAGGAAGAGACGTGGCGCAGACACCAGCATCGGAATCATTGGCC11.36986301369863No Hit
TACCAGCACTGCCATTGATGTGCTCATGATGATCTTTGTGGTCATTCTCT11.36986301369863No Hit
TCCGAACACAACCCAAGTTGATGTCGTGTTGCAGCAGCTATCTACATCAT11.36986301369863No Hit
GATGGAAGGCTGATAACCGCCAACCCCGTGATTACTGAAAGCACTGAGAA11.36986301369863No Hit
CAGTGCATGGCTCCCAGCATAGCGGGATGATTGTCAATGATACAGGATAT11.36986301369863No Hit
ACTGAAAGCACTGAGAACTCAAAGATGATGTTGGAGCTTGACCCACCATT11.36986301369863No Hit
GGTGAGTAACATGGCCGAGGTAAGATCCTATTGCTACGAGGCATCGATAT11.36986301369863No Hit
GAATCACAAGCACTCCAAGAGAGAAGTGGTCCATATGATCGGTTGACCCC11.36986301369863No Hit
AATGCAGACCCTGACCCCAGTTGGATGGCTGAAAACCGCCAACCCAGTGA11.36986301369863No Hit
ATTAGAATGTGACCCAGCCGTCATAGGAACAGCTGTTAAGGGAAGGGAGG11.36986301369863No Hit
GGCTTCAACATCATTATAGATGAATACCCCCGTGCCACATCTCGTTTCCT11.36986301369863No Hit
GGAAGAGGGGATCTGTGGGATCTCATCCGTTTCAAGAATGGAAAACATCA11.36986301369863No Hit
GGAAGGGTCATTGAGGAATGGTGCTGTAGGGAATGCACAATGCCCCCACT11.36986301369863No Hit
TTTTTACAGATCCCACAACAACTGTCAGTTGAACTCCATTCTCCTCTAGG11.36986301369863No Hit
AATCAAGAGAATACACGAAGCACTTGATCAAGGTTGAAAACTGGATATTC11.36986301369863No Hit
GCATAGGGCACTCAAAACTCATGGTGGCGTCACACATGTGCCCGAGGTCC11.36986301369863No Hit
TTCTAATGAGTAATCTTCTCTGACCTTAAGCCAGACACTGGTGTGGAAGA11.36986301369863No Hit
ATTGATGTGCTCATGATGATCTTTGTGGTCAATCTCTTCTTCAACCCCTC11.36986301369863No Hit
CTCACACATTGTGGACAGATGGAGTAGAAGAAAGTGATCTTATCATACCC11.36986301369863No Hit
GAACAGCTGTTAAGGGAAGGGAGGACGCGCACAGTGATCTGGGCTATTGG11.36986301369863No Hit
GATCTATCTCCCTCACATGCTTGGCCCTGGGGGGAGTGATGATCTTCATC11.36986301369863No Hit
GACTACAGCCATGGCAGCAGAGCTCACTAGACGCGGGAGTGCATACTACA11.36986301369863No Hit
GAATAATCAATGCTAGGAAAGAGAGGAAGAGCCGTGGCGAAGCCACCAGC11.36986301369863No Hit
CCAGTGTGGAGTTCCGGTGTCTGCCCCAGCATGCCAAGGCAATGGGATGT11.36986301369863No Hit
GGTCATGATAATGCTGATTGCCCCGGCATACAGTATAAGGTGCATTGGAG11.36986301369863No Hit
CCCTTCCACTTGCAGTAGTTGATCTCAGAGATGGTCCTCTAGTTCCGCAT11.36986301369863No Hit
CCTGCACTGTGACTGGTCCATGCAGTGTTTCAGCTGGCTCTTTTGAGCAT11.36986301369863No Hit
ACAGTATCAGGTGCATTGGAGTCAGCAATAGAGACTTCGTGGAGGGCATG11.36986301369863No Hit
CATGTGTGACGCCACCATGAGTTATGAGTGCCCTATGCTGGATGAGGGAG11.36986301369863No Hit
GCACTGAGAACTCAAAGATGATGTTGGAGCTTGACCCACCATTTGGGGAT11.36986301369863No Hit
GAGCTGCCCCATGGCTGGAAAGCCTGGGGGAAATCGTATTTAGTTAGGGC11.36986301369863No Hit
CTCTGCATGTCCACCGCCATCTGGACTGGGATCTTGCAGGGTCCATCTGT11.36986301369863No Hit
TATCAGTGCATGGCTCCCAGCATAGCGGGATGATTGTCAATGATACAGGA11.36986301369863No Hit
TTGTGGACAGATGGAGTAGAAGAAAGTGATCTTATCATACCCAAGTCTTT11.36986301369863No Hit
ATCCAATAGCCCAGATCACTGTGCGCGGCCTCCCTTCCCTTAACAGCTGT11.36986301369863No Hit
CTTCCCGGTCATCTTCTTAGAACACGTAAACTTGGCACATGTCACCAAGC11.36986301369863No Hit
CTCAAAGATGATGTTGGAGCTTGACCCACCATTTGGGGATTCTTACATTG11.36986301369863No Hit
ATCTAGAAGAGCCGTGACGCTCCCTTCTCACTCTACAAGGAAGTTGCAAA11.36986301369863No Hit
TGGAAAACATCATGTGGAAATCAGTAGAAGGGGAGCTCAATGCTATCCTA11.36986301369863No Hit
GTAGAAGGGGAGCTCAATGCTATCCTAGAGGAGAATGGAGTTCAACTGAC11.36986301369863No Hit
GTTGAACTCCATTCTCCTCTAGGATAGCATTGAGCTCCCCTTCTACTGAT11.36986301369863No Hit
TCCATGCTCTGTGCTCACGCGGCCATTCCTTCCGTCTCTCACCGTCGACA11.36986301369863No Hit
GAATAGTTTTCTTGTGGAGGATCACGGGTTTGGAGTCTTCCACACCAGTG11.36986301369863No Hit
GCAATAGGATCTTACCTCGGCCATGTTACTAACCTGTCTCTTAAAAACAT11.36986301369863No Hit
GTGTGGAGTTCCGGTGTCTGCCCCAGCATGCCAAGGCAATGGGATGTCAT11.36986301369863No Hit
CTACTCCATCTGTCCACAATGTGTGAGACTTTGGCCATTCACATGTTTTC11.36986301369863No Hit
TCATAGGCACGCTGCTAGTGTGGTTAGGTTTGAACACAAAGAATGGATCT11.36986301369863No Hit
GTCTTGGAACATGGAGGCTGCGTTACCGTGATGGCACAGGACAAGCCAAC11.36986301369863No Hit
ACCATTGACCTCACTAAGTTGCTCTCTGGTTCTTTCCTGGGCCTTATCTC11.36986301369863No Hit
AGGTAGGACTCACCTTGTGTTGGGCAACGTCTGTCCGAAGCCATGTCCGA11.36986301369863No Hit
AACACAAGGTGAGGACTACCTTGACAAGCAATCAGACACTCAATATGTCT11.36986301369863No Hit
GGTTTACGTGGAGGAGACATGCGGAACTAGAGGACCATCTCTGAGATCAA11.36986301369863No Hit
ATCCAGCATAGGGCACTCATAACTCATGGTGGCGTCACACATGTGCCCGA11.36986301369863No Hit
CATTCTTTGTGTTCAAACCTAACCACACTAGCAGCCTGCCTATGAGGATC11.36986301369863No Hit
TTACCTGACCATGAACAATAAGCATTGGTTGGTGCACAAAGAGTGGTTTC11.36986301369863No Hit
GGTTTGGAGTCTTCCACACCAGTGTCTGGCTTAAGGTCAGAGAAGATTAC11.36986301369863No Hit
ATGACACAGGATATGAAACTGACGAAAATAGAGCGAAAGTCGAGGTTACG11.36986301369863No Hit
TTTTTAGGCGGCATTTCAAGTGGCCAGAGAACAGCTTTCCCTTTGCACCA11.36986301369863No Hit
GTCAATGATACAGGATATGAAACTGACGAAAATAGAGCGAAAGTCGAGGT11.36986301369863No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGAGT50.0144.01
GTGAGTA50.0144.02
GAGTAAC50.0144.04
AGTAACA50.0144.05
TAACATG50.0144.07
GTAACAT50.0144.06
TGAGTAA50.0144.03
AACATGG50.0144.08
ACATGGC100.072.09
Produced by FastQC (version 0.11.2)