FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_blood_ifn--_red_needle_f12_pcr1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_blood_ifn--_red_needle_f12_pcr1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906739
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTC230172.5384371908564645No Hit
GACAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAACAT170851.8842246776635836No Hit
CTGACAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAAC130201.435914855322204No Hit
GCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATG98331.0844355431937966No Hit
TGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTG95821.0567539280873548No Hit
ACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAACATCGTG86360.9524240161722393No Hit
GTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAG84440.9312492348955984No Hit
GATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGT82880.9140447251083277No Hit
CATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAACATCGTGC81790.9020236253210681No Hit
CGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTG66600.7345002255334776No Hit
GCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCA59050.6512348095758537No Hit
GTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGC57290.6318245934055996No Hit
GTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGG51040.5628962689373679No Hit
TGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCA50860.5609111331926828No Hit
CACTGACAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACA38580.42548076127750106No Hit
ATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAACATCGTGCC31930.35214102404330244No Hit
GCACTGACAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGAC31230.34442105170286047No Hit
GCCACGCACTGACAACATCGTGCCACGCACTGACAACATCGTGCCACGCA30830.3400096389368936No Hit
GTGCCACGCACTGACAACATCGTGCCACGCACTGACAACATCGTGCCACG30020.3310765280858108No Hit
TTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGT29630.32677540063899313No Hit
GGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGAT27340.301520062553833No Hit
CGCACTGACAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGA25480.2810069931920873No Hit
CAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAACATCG23930.2639127687239658No Hit
TGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGA21700.23931914255370068No Hit
ACTGACAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAA21490.2370031508515681No Hit
TGACAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAACA18920.20865982383023118No Hit
ACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTT18510.2041381257451152No Hit
GTATTACCTGACCATGAACAATAAGCATTGGTTGGTGCACAAAGAGTGGT15820.17447137489398823No Hit
ATGCTGGATGAGGGAGTGGAACCAGATGATGTCGATTGCTGGTGCAACAC15280.168515967659933No Hit
GGTAATACAGATCTGAAAAGTCAAGGCCTGTCCTTGGTTCACAGTCAAGT15090.16642054659609876No Hit
CCTTAAACCTGTCTTGTAACCTTGATACTTACTTTCAGCCTCCATGTGTC15060.16608969063865126No Hit
CTACATGTACTTGGATAGGAGCGATGCCGGGAAGGCCATTTCGTTTGCTA14490.1598034274471485No Hit
GGTTTAAGGAGACCAATAGAAACTGGGCTTGTCGAGACAGAGAAGACTCT13620.15020860468117064No Hit
AGTCTGAAGCGAGAGCTAACAACAGTATCAACAGGTTTAATTTGGATTTG13200.1455766212769055No Hit
GTATATGACTTTTTGGCTCGTCGAGCTTCCCAAAAGCCAGGCAATGGCAA13180.14535605063860713No Hit
ATCTAAGCTTGTCCATTTTTAGGCGGCATTTCAAGTGGCCAGAGAACAGC12860.14182692042583367No Hit
TCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAACATCGTGCCA11540.12726925829814315No Hit
GAATATGACACGCCCTTCAATCTAAGCTTGTCCATTTTTAGGCGGCATTT11430.12605611978750225No Hit
TCCGTACACAACCCAAGTTGATGTCGTGTTGCACCAGCAATCGACATCAT11270.12429155468111552No Hit
GAGTTATGAGTGCCCTATGCTGGATGAGGGAGTGGAACCAGATGATGTCG11260.12418126936196633No Hit
GTCATAGGAGTTGGGGACAAGAAAATCACCCACCACTGGCATAGGAGTGG11080.12219613361728127No Hit
TTCATATCCTGTATCATTGACAATCATCCCGCTATGCTGGGAGCCATGCA10750.11855671808535863No Hit
TCCTTGTAGAGTGAGAAGGGAGCGTCACGGCTCTTCTAGATCGCCGTGCC10660.11756415021301608No Hit
TCTCACAGTGGCCTCAAATGCCTTTCCGATGGTGCTACCACTCCTATGCC10110.1114984576598117No Hit
TCCTAGAGGAGAATGGAGTTCAACTGACAGTTGTTGTGGGATCTGTAAAA9920.10940303659597746No Hit
TCTTAGAACACGTAAACTTGGCACATGTCACCAAGCTCCCTTTGCCAAAA9900.10918246595767911No Hit
GTTAGTAACATGGCCGAGGTAAGATCCTATTGCTACGAGGCATCGATATC9590.10576362106405482No Hit
ACTCTATGTCAACTGTTGGCTTGTCCTGTGCCATCACGGTAACGCAGCCT9550.10532247978745812No Hit
TCCCTTAACAGCTGTTCCTATGACGGCTGGGTCACATTCTAATGAGTAAT9520.10499162383001061No Hit
GTACAGATCATGGACCTCGGGCACATGTGTGACGCCACCATGAGTTATGA9510.10488133851086145No Hit
GTGCGTGGCACGGCCATTGCTCGAATTGCCAACCAGGCCAAAGCAAATCC9500.10477105319171229No Hit
GTAGTATGCACTCCCGCGTCTAGTGATCTCTGCTGCCATGGCTGTAGTCA9490.10466076787256309No Hit
GGATAGGAGCGATGCCGGGAAGGCCATTTCGTTTGCTACCACATTGGGAG9200.10146249361723716No Hit
CTCAATATGTCTGCAAAAGAACATTAGTGGACAGAGGTTGGGGAAACGGT9070.10002878446829792No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAAGC2800.077.1407854
AATGTTG1500.076.806411
AGTCTGA2850.075.795811
TCTGAAG2850.075.7874453
GTCTGAA2850.075.7874452
GAAGCGA2950.073.218386
ACACGAT1650.069.824011
ATGCGTG1400.066.8627241
AAGCGAG3300.065.452797
TCGAGTC1650.065.452793
CGAGTCT1700.063.527714
TTCGAGT1850.058.3768122
TGAAGCG3850.057.972475
TGAGCCA400.00621929653.998555
GAGTCTG2050.052.681525
CCTTGGA1850.046.701452
CGACAGT700.001093323941.1417542
TGTTGCC700.001093323941.1417545
CTGTCTT10950.029.5882479
CCTTAAA11000.028.8024041
Produced by FastQC (version 0.11.2)