FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_3036_d8_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_3036_d8_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169163
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT4210.2488723893522815No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG2580.15251562102823904No Hit
GGCTGCTGCCTGTGGTGAAGGCTCATCAGGGAGGAGATAGGGCGAAGGGA2520.14896874612060557No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA2170.1282786424927437No Hit
GAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCA1920.11349999704427091No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC1890.11172655959045417No Hit
CCCACTTCCCCTGCTTTTGCTCCCCCCAGGGGACCCGGACAGGTCCAGCC1880.11113541377251526No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT1730.10226822650343159No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGTG100.0072338204143.999988
GAGCAAA4750.086.41
CAAAAGC6150.066.731714
GCAAAAG6550.062.656493
AGCAAAA6650.061.7142872
CGAAAGC1600.058.4999963
AGCGAAA1500.057.61
AAAAGCA8400.049.7142875
CGTTACA450.00989217148.0000043
CCTATGG450.00989217148.0000042
GCGAAAG1850.046.70272
ACCATAG657.573022E-444.307698
AAAGCAG10250.044.253666
GCAGGTA1401.4908437E-841.142869
CCATAGA750.001531091838.3999989
AAGCAGG12700.037.417327
TAGAAAC1355.214042E-737.3333323
GAGCGAA1209.979338E-636.01
CTACTGG800.002102043535.9999961
TATAGAC1459.12245E-734.758622
Produced by FastQC (version 0.11.2)