FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_3036_d3_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_3036_d3_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372645
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA21590.579371788162997No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG20750.5568302271598975No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG11860.31826537321042814No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT8280.22219538703055186No Hit
TCCACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT7940.21307142186263064No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT7500.20126393752767377No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC6250.16771994793972816No Hit
GTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATC5610.1505454252707No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC5460.14652014652014653No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG5390.14464168310322156No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT5160.13846958901903955No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT4950.13283419876826472No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG4770.12800386426760055No Hit
GAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT4580.1229051778502328No Hit
AACTCATCCTTTATGACAAAGAAGAAATAAGGAGAGTTTGGCGCCAAGCA4390.11780649143286505No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC4320.1159280280159401No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA4270.11458626843242228No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC4170.11190274926538664No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG4140.11109769351527593No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC4030.10814582243153673No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT4000.10734076668142603No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC3840.10304713601416897No Hit
AGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTAT3790.10170537643065115No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC3780.1014370245139476No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT3780.1014370245139476No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG151.263082E-4144.01921
CGTCAGA151.2637574E-4143.999862
GAGCGAA1100.0130.926531
TTATTGG203.972E-4107.99993
TTATAGG850.093.1888961
GTCAGAT259.6436264E-486.399913
GCAGGTA4350.081.103379
GCAGGGT7300.079.890349
AGCGAAA2550.076.245451
CCTACTA2000.075.599925
TCCACCT1950.073.8561
CACCTAC2100.071.999943
GCGAAAG2750.070.690842
CCACCTA2050.070.243842
CGAAAGC2800.069.4285053
GCAAAAG17350.069.302533
GAGCAAA17100.068.219621
AAGCAGG21400.063.2522747
AGCAGGG13250.061.4037138
AGCAAAA19050.061.2282872
Produced by FastQC (version 0.11.2)