FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_3035_d8_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_3035_d8_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33235
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG1900.5716864751015496No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA1390.4182337896795547No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC760.22867459004061982No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG720.21663908530163983No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG580.17451481871520988No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA530.1594704377914849No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA460.13840830449826988No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT450.13539942831352492No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT450.13539942831352492No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC450.13539942831352492No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT440.1323905521287799No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT420.1263727997592899No Hit
GGTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGC410.12336392357454491No Hit
TCCACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT410.12336392357454491No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC390.11734617120505492No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC370.11132841883556492No Hit
GGCAAGTGCACCAGTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGGA360.10831954265081992No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC151.253488E-4144.000021
AATTGTG100.0072078314144.07
GCAGGTA259.5658394E-486.3999949
ATATACT300.00197266272.000015
GCAAAAG1300.072.03
GAGCAAA1500.072.01
AGCAGGT554.1425646E-665.4545448
CGAAAGC350.003634504761.7142833
GCGAAAG350.003634504761.7142832
GACAATA350.003634504761.7142831
GAATAAG350.003634504761.7142831
TCCTACA350.003634504761.7142832
AGCGAAA350.003634504761.7142831
GAGGCTA350.003634504761.7142839
AGCAAAA1600.058.52
CAAAAGC1650.056.7272764
GCAGGAG400.00616621654.09
AAGCAGG2100.051.428577
AAAAGCA1850.050.5945935
GCAGGGT1004.7744834E-850.3999989