FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_3034_d8_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_3034_d8_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330047
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA4670.14149499919708405No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT4560.13816214054361955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA11350.087.5405651
GCAAAAG13850.072.2588653
AGCAAAA13950.072.2572
CAAAAGC14700.068.080634
TCGCTAG350.003663666861.7133759
AAAAGCA18500.054.4856835
AGCGAAA2400.053.9992072
AAAGCAG22550.051.085726
GAGCGAA2550.050.822781
GCGAAAG2900.047.171723
CGAAAGC3000.045.599334
TAGACCT2250.041.599394
AAGCAGG30250.038.3200957
ATAGACC2550.036.705343
TATAGAC2550.036.705342
GATGCGT850.002834070733.8818556
GCCTACA950.00488569530.315341
ATGCGTG950.00488569530.315347
GTATAGA4250.028.7995741
GTATAAT1555.7550198E-527.8705581
Produced by FastQC (version 0.11.2)