FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_3033_d8_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_3033_d8_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464838
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG9810.2110412659894415No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA7940.17081219693742766No Hit
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC6360.1368218605191486No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT5560.11961156359850099No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC5120.1101459002921448No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG5020.10799461317706383No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA14250.084.375541
GAGCGAA3450.075.12741
AGCAAAA18100.066.8260352
GCAAAAG19700.061.3985373
CAAAAGC20150.060.7419664
AGCGAAA4750.057.5976752
CGAAAGC5300.051.620564
GCGAAAG5300.051.620563
AAAGCAG30200.049.5874066
AAAAGCA25900.047.5347635
AAGCAGG38150.039.4427077
ATAGACC3150.036.5699543
TAGACCT1708.300049E-833.8809854
GCAGGTA4050.031.9987099
GCAGGGT8900.031.5492889
CGTTACA1453.656047E-529.79193
GTATTAC3201.8189894E-1229.2488211
GATAGAC1907.5415664E-626.5252462
AGCAGGG28500.026.5252448
AGCAGGT10200.026.1165948
Produced by FastQC (version 0.11.2)