FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_3033_d1_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_3033_d1_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326212
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA14120.4328473508025456No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG13250.40617757777151053No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG9660.2961264453790787No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG6200.19006045148553702No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT6020.18454256741015046No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT5790.17749193775826763No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT4990.15296800853432738No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA4320.13242921780927738No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC4200.12875062842568635No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA3820.11710176204431474No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA3550.1088249359312349No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC3500.10729219035473864No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG3440.10545289566294312No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT3420.10483979743234462No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT3380.10361360097114759No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT3380.10361360097114759No Hit
GAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT3320.10177430627935208No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC3300.10116120804875357No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA850.0135.563321
CCACCTA550.0130.921772
TCCACCT600.0120.030021
CCTACTA600.0120.011635
CACCTAC600.0120.011633
TCTACAC1000.093.609063
TAGCAAA406.3671905E-789.994928
CTACACT1100.085.099154
GCAGGTA3950.082.020699
GCAGGGT5200.081.6877069
CAGGTAC755.820766E-1176.807443
AGGTACT755.820766E-1176.807444
GCAAAAG13000.074.222583
AGCGAAA1850.073.964451
TTATAGG1400.072.018021
GGATAGC502.3980192E-672.006975
GATAGCA554.2230804E-665.4608846
AAGCAGG17600.062.1783147
GAGCAAA15550.062.0605431
GCGAAAG2250.060.8058932
Produced by FastQC (version 0.11.2)