FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_3032_d8_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_3032_d8_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439046
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC7580.17264705748372608No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT7360.16763619301849922No Hit
CCCAATATCACCCCCACCATGCAGGTCCTTCTCCAAGGAGTTCACCACGG5690.12959917639609517No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA5060.11524988270021821No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG4880.11115008450139621No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG4600.10477262063656201No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA4510.10272272153715101No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC4470.10181165527074611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA14250.091.996241
GCAAAAG19700.068.7083
AGCAAAA19400.067.9148942
CAAAAGC20300.065.967894
AGCGAAA3850.057.9982071
TACGGTG400.00621746153.997914
AAAGCAG29450.052.5616266
GAGCGAA3900.051.7138671
AAAAGCA26300.051.4656835
CGAAAGC4950.047.998143
GCGAAAG5150.046.1341322
GCAGGTA3900.044.3059739
CGTTACA1755.638867E-1141.1412623
AAGCAGG41050.037.8840377
GTTACAC2104.0563464E-1034.2843864
AGCAGGT10250.033.7157678
TATACTC900.00375172231.998765
TATAGAC3900.029.5373172
GCTTTCG2252.9927833E-828.812011
TTTCGCT2253.006062E-828.7988823
Produced by FastQC (version 0.11.2)