FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_3032_d3_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_3032_d3_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206020
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA11060.5368410833899622No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG10560.5125715949907776No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG5750.2790991165906223No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT3820.1854188913697699No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT3670.17813804485001455No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT3600.17474031647412872No Hit
TCCACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT3470.16843024949034074No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC3050.1480438792350257No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA3020.14658770993107464No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA2790.13542374526744977No Hit
GTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATC2660.12911367828366177No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC2620.12717211921172702No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG2530.1228036112998738No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT2500.12134744199592273No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG2380.11552276478011844No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG2380.11552276478011844No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC2360.11455198524415106No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC2340.11358120570818368No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT2320.1126104261722163No Hit
GAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT2250.10921269779633044No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT2160.10484418988447723No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC2130.10338802058052615No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT2110.10241724104455878No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG2080.1009610717406077No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA950.0121.280531
TTATAGG458.913048E-11112.0160451
GCAGGGT3550.089.230569
AGCGAAA1550.074.333232
CAGGTAC300.001986239372.0103153
CGAAAGC1800.068.009744
GCAGGTA1600.067.4932869
AAGCAGG10150.067.382467
AGCAGGG6150.066.725078
CGGAATA651.4594298E-766.471062
TACTGCC651.4618854E-766.4549267
GCGAAAG1850.066.1716463
ATACTGC702.4369365E-761.723136
TCGGAAT702.4369365E-761.723131
GACTAAC350.00365942161.723131
CTGCCCG702.4410292E-761.7081459
GCAAAAG9300.059.6214453
GAGCAAA9450.058.6750761
TGCCATG1251.8189894E-1257.5942738
AAAGCAG12700.054.4336
Produced by FastQC (version 0.11.2)