FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_3032_d-2_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_3032_d-2_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127118
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT1650.12980065765666546No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA1570.12350729243694836No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT1410.11092056199751411No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1250.086.3999941
GCGAAAG1350.085.3333362
CGAAAGC1650.069.8181843
GAGCGAA400.00620639354.01
GTATTAC1553.6452548E-837.161291
GAGCAAA2401.8189894E-1236.01
TACATCA1751.0599433E-732.9142885
GTTTATA1352.23071E-532.01
TTACATC1601.963348E-631.54
GCAAAAG2809.094947E-1230.8571413
CAAAAGC3550.030.4225354
TACTTAC950.004874169430.3157887
GTTAAAG950.004874169430.3157881
TATTACA1952.738161E-729.538462
GTATTAA1753.9477072E-628.8000011
TAGAGTG1255.340872E-428.85
TATATAC1306.7180203E-427.6923073
CTACTGT1050.0079474727.4285726
TTACTAC1050.0079474727.4285724
GAAAGCA4500.025.64
Produced by FastQC (version 0.11.2)