FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_3031_d8_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_3031_d8_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80310
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG1870.2328477151039721No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA1580.19673764163865023No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA1100.13696924417880713No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC1000.12451749470800647No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG960.1195367949196862No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG840.10459469555472545No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGCG100.0072267926144.09
GAGCAAA2100.082.285721
GAGCGAA353.537378E-582.285711
AGCGAAA608.327697E-872.01
GCAAAAG2750.062.8363653
AGCAAAA2850.060.631582
CAAAAGC2850.060.631584
GCGAAAG851.3573299E-859.2941172
TCTAATA400.006198100754.02
CGAAAGC953.2643584E-853.052633
GCAGGTA953.2643584E-853.052639
TAGCCCT450.00987339748.0000049
AGTAGAA450.00987339748.0000041
AAGCAGG4800.043.57
AAAAGCA4000.043.1999975
AAAGCAG5100.039.529416
GTAGAAA1001.4374251E-436.01
AGCAGGT2009.344149E-932.3999988
GTACTGA1153.2663756E-431.3043486
GGATAGA950.00486359430.3157881
Produced by FastQC (version 0.11.2)