FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_3031_d-2_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_3031_d-2_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256657
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT6800.26494504338475083No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA6560.25559404185352436No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG5140.20026728279376754No Hit
GGTCTATACTACCCAAAATCTACATATTTGATGTAATACCTATCAAAATA4290.1671491523706737No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACCTCTTAACAATA2580.10052326646068488No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG2400.089.997442
CGAAAGC2350.088.848543
AGCGAAA2850.073.2896351
AGCGTGC400.00621427653.9984668
TCAAGCG400.00621427653.9984665
CAAGCGT450.00989907347.998646
GCGTGCG450.00989907347.998649
GATAGGC450.00989907347.998644
TAGACCT3650.047.341124
ATAGACC4050.044.4431843
TATAGAC4750.042.4409032
AGACCTC4350.039.7230075
CTGCCGT800.002104360135.9989789
CCTCTTA4800.035.9989748
GCAAAAG3100.034.837723
GACCTCT5150.033.5524446
GAGCAAA3600.032.0115621
TATGGGC900.00374865231.9990924
ATTGTAC900.00374865231.9990928
GCGCCCT900.00374865231.9990929
Produced by FastQC (version 0.11.2)