FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_3030_d-2_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_3030_d-2_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224606
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT4100.1825418733248444No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA3540.15760932477315834No Hit
GGTCTATACTACCCAAAATCTACATATTTGATGTAATACCTATCAAAATA3400.15137618763523683No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG2630.11709393337666849No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA4700.079.655511
GAGCGAA1550.074.318791
GCTTTCG608.3993655E-871.996321
TCGCTCC755.1059033E-967.196575
AGCGAAA2400.056.997091
TTTCGCT806.1416904E-753.997253
TTCGCTC806.1416904E-753.997254
CGAAAGC2600.052.6126983
CAAAAGC8000.049.4974754
GCAAAAG8100.048.886393
GCGAAAG2800.048.854652
CTTTCGC901.383869E-647.997552
AGCAAAA8100.047.1087042
AAAGCAG14150.036.6341746
AAAAGCA11150.036.159595
TAGAGTA800.00210403535.9981654
TGTATAC900.003748077231.9983672
TATAGTG900.003748077231.9983675
GTATATA5100.028.2338521
TTATAGT1306.736229E-427.6908934
Produced by FastQC (version 0.11.2)