FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_3029_d-2_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_3029_d-2_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481467
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT8010.16636654225523245No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA5930.1231652428930747No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG4980.10343388020362766No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA4840.10052610043886706No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4250.096.574661
CGAAAGC4650.089.806393
GCGAAAG4800.086.999942
GTGCGTA400.00621433554.0055731
GAGCGAA1650.052.3690381
GAGCAAA9650.035.8171651
CAAAAGC9600.034.4999734
GCAAAAG10250.033.014613
ATAGACC2404.7293724E-1132.9999773
GGAGCGA1402.8745371E-530.8603251
CCGATAC1652.5151803E-630.5454319
ACTATCC1000.006280157228.799988
AGCAAAA11900.028.4369542
AAAGCAG20600.028.3106585
GGTCTAT2054.2795E-728.100461
CCTAGAC1306.7455304E-427.6922873
TATAGAC6050.027.3718812
ACCGATA1907.539982E-626.5262978
TAGACCT2757.2632247E-926.1817994
GAAAGCA16350.025.9816344
Produced by FastQC (version 0.11.2)