FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_3028_d8_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_3028_d8_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145957
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG3900.2672019841460156No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA3540.2425371856094603No Hit
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC3290.22540885329240803No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT2790.19115218865830347No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA2200.1507293243900601No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG2060.14113745829251081No Hit
CCCAATATCACCCCCACCATGCAGGTCCTTCTCCAAGGAGTTCACCACGG2050.14045232499982874No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC2010.13771179182910034No Hit
GAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCA1800.12332399268277644No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC1500.1027699939023137No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA5250.091.881571
GAGCGAA1500.091.195881
AGCGAAA1450.079.444692
AGCAAAA6200.075.480462
CCAATAT1250.074.876622
ATATCAC1250.074.876625
GCGAAAG1600.071.996743
CGAAAGC1650.069.8150254
GCAAAAG6600.069.8150253
CAAAAGC6950.066.2991644
CATTATA350.003660017661.7114941
AAAGCAG9450.060.9496236
TATCACC1500.057.5973976
AAAAGCA8100.056.8863145
AGCAGGT3250.050.9515468
ATAGACC859.3086055E-750.8212363
AAGCAGG11650.049.4398277
TAGACCT752.6465606E-547.9978334
CTATAAT450.0098912447.9978331
ATCACCC1950.044.305697
Produced by FastQC (version 0.11.2)