FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_3028_d5_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_3028_d5_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282051
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG15920.5644369280732917No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA14080.49920049920049914No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT12610.44708226526408346No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA12020.4261640625276989No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG8790.31164576619122075No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT5650.20031838213656394No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT5620.19925474470929017No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC5230.18542745815473088No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA4180.1482001482001482No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC4050.14359105268196176No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT3940.13969104878195787No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG3870.13720922811831904No Hit
TCCACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT3870.13720922811831904No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG3850.1365001365001365No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT3710.1315364951728588No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC3590.12728194546376365No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT3480.12338194156375974No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC3450.12231830413648595No Hit
GTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATC3380.11983648347284712No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA3360.11912739185466457No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC3260.11558193376375195No Hit
ACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTT3220.1141637505273869No Hit
GAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT3170.11239102148193057No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC3170.11239102148193057No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG3150.11168192986374804No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA3050.1081364717728354No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC2970.1053001053001053No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG2960.10494555949101404No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1600.0121.515421
TTATAGG451.14141585E-896.012181
GAGCAAA13550.083.966371
GCAGGGT5500.079.850529
TCTAGCT755.820766E-1176.796132
GCAGGTA3750.074.876229
AGCGAAA2600.074.765462
AAGCAGG18000.073.996277
CGAAAGC2650.073.354784
GCGAAAG2650.073.354783
AGCTCTA801.05501385E-1071.996375
TGCCATG851.8007995E-1067.7612848
GCAAAAG16950.066.68693
AGTAGAA1650.065.462851
AGCAAAA17500.065.0024262
AGCAGGG8300.064.189538
GGATAGC902.9831426E-1063.9967735
GATAGCA902.9831426E-1063.9967736
AGGTCGT808.514689E-962.9968226
TCCACCT1500.062.4079171
Produced by FastQC (version 0.11.2)