FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_3026_d8_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_3026_d8_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512453
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG8920.17406474349842815No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA7230.14108610936027305No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC7130.13913471089055973No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA6840.1334756553283911No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG6310.1231332434389105No Hit
GAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCA5640.11005887369183126No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA14350.080.2779851
AGCAAAA17400.066.206272
GCAAAAG18650.062.1549033
CAAAAGC20950.055.3312154
GAGCGAA3300.052.363141
AGCGAAA3650.045.3694342
AAAAGCA27650.042.4444435
AAAGCAG31500.042.0567446
GCGAAAG4300.040.1856652
CGAAAGC4650.037.1609343
GCAGGTA5050.035.6432279
AAGCAGG39050.034.1097957
GACCGCC850.00283545433.882037
GTCTAAG1102.5355731E-432.7269631
GTACTAT4650.032.515821
AGCAGGT11750.030.0252488
CTATAGG1753.9788756E-628.7997261
CGTTACA2156.496284E-726.7904433
CAATCGC3505.456968E-1226.7426035
ATCGCAC3607.2759576E-1225.9997527
Produced by FastQC (version 0.11.2)