FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_3025_d8_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_3025_d8_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1059270
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC15130.14283421601669075No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT13530.1277294740717664No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA33450.089.772971
CAAAAGC44100.069.063084
AGCAAAA45050.067.127222
GCAAAAG48350.063.439143
CGAAAGC9950.054.9964523
AGCGAAA10100.054.900341
GCGAAAG10800.052.0013962
AAAAGCA61750.050.0223965
GAGCGAA7500.048.008091
AAAGCAG71700.047.2983446
AAGCAGG87050.039.0377
CAATTCG1000.00628556528.7980569
AGCAGGG68650.028.0010228
TATAGAC7800.026.7699492
GATACTG10850.025.8845461
GCTTTCG4200.025.718621
GCAGGGG40450.025.6299139
CAATACG1751.3159613E-424.6863784
GTATACG1500.001555533524.0006453
GGCGGTT1550.001883588623.2242377
Produced by FastQC (version 0.11.2)